| WARF: P02052US |  |
| Modified Acyl Carrier Proteins |
| INVENTORS | • | Brian Fox, Jeffrey Haas |
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing acyl carrier proteins modified with a non-radioactive label.
OVERVIEW
Acyl carrier protein (ACP) is a small protein that functions as an acyl carrier. It is involved in fatty acid biosynthesis and many other important biological processes in both eukaryotes and prokaryotes. ACP is difficult to obtain in a functional state from recombinant systems, primarily because posttranslational modification is required for proper protein functioning.
THE INVENTION
UW-Madison researchers have developed purified apo-, holo- and acylated-acyl carrier proteins modified with a non-radioactive label that is covalently bonded to a modified tyrosine residue within the protein. To generate the labeled proteins, an amino group is first introduced into a tyrosine residue of apo-ACP. Next, a fluorescent molecule is covalently bonded to the amino group. The labeled apo-ACP is then converted to holo-ACP and subsequently to acylated-ACP.
APPLICATIONS
- Investigating reactions involving or mediated by ACPs
KEY BENEFITS
- Label is catalytically silent -- it does not interfere with binding of enzymes to ACP molecules.
- Addition of label to tyrosine residue is very specific.
- Enables interactions of ACPs to be examined in greater detail, with greater ease, and with greater precision than current radiolabeled ACPs
- Involves no time-consuming and waste-generating radioactivity
ADDITIONAL INFORMATION
For More Information About the Inventors
Intellectual Property Status
Tech Fields
Research Tools - Synthesis & purification
CONTACT INFORMATION
For current licensing status, please contact our team at
licensing@warf.org
or phone 608.262.4924. (Clicking this link will open a contact form in a popup window. If you have problems viewing the form, try disabling your popup blocker software.)
Since its founding in 1925 as the patenting and licensing organization for the University of Wisconsin-Madison, WARF has been working with business and industry to transform university research into products that benefit society. WARF intellectual property managers and licensing staff members are leaders in the field of university-based technology transfer. They are familiar with the intricacies of patenting, have worked with researchers in relevant disciplines, understand industries and markets, and have negotiated innovative licensing strategies to meet the individual needs of business clients.
