Diagnostic Assays

Diagnostic Assays Portfolios

Most Recent Inventions

Enhanced Endotoxin Detection: New Advantages in Liquid Crystal Assays for Gram-Negative Pathogens

UW–Madison researchers have now demonstrated enhanced endotoxin detection in the presence of masking agents in their previous liquid crystal system.

Unlike the LAL assay, the LC-based method does not suffer from LER or any loss of sensitivity due to the presence of cations (e.g., Ca2+ or Mg2+), buffers (e.g., citrate), surfactants (e.g., SDS), chelating agents (e.g., EDTA), proteins or nucleic acids (e.g., DNA or RNA). Thus, the LC-based method provides faster and cheaper detection of endotoxin when compared to existing methods, such as the LAL assay.

Predicting Glucoregulatory Dysfunction

UW–Madison researchers have developed a method based on blood lipid chemistry to identify a subject at risk for glucoregulatory dysfunction. The method involves obtaining a biosample from the subject, separating the diacylglycerol fatty acids and determining if the concentration is above or below a control range.

Field Test for Mycobacterial Infections

A UW–Madison researcher has identified a set of biomarkers that can indicate whether a mammal is vaccinated or infected, as well as the type of infection (bovine tuberculosis or Johne’s disease).

Assay to Determine Risk of Fungal Infection

More specifically, the researchers have developed a detection agent made up of calnexin peptides that recognize the telltale signs of infection. The peptides are able to track how a patient’s helper T cells respond to infection and/or vaccination.

Colorimetric Presumptive Illicit Drug Detection

Researchers at the University of Wisconsin – Platteville have developed a rapid, inexpensive color test for the presumptive identification of multiple classes of controlled substances including synthetic cathinones, cannabinoids, opiates and stimulants including cocaine. This novel, aqueous reagent test can be delivered in an industry-accepted plastic test pouch containing an ampoule of chemical reagents necessary for preliminary testing of controlled substances. Test reagents can be tailored to detect specific target compounds of interest and as new derivatives are developed.

Most Recent Patents

Diagnostic Kit for Blastomycosis

UW–Madison researchers have developed a method for obtaining highly pure native BAD-1 protein that could be used to detect B. dermatitidis infection.

A solution containing native BAD-1 protein or fragments is collected from cultured fungus strains. The solution is combined with nickel-chelating resin, washed and eluted to obtain a highly pure form without the need for more expensive recombinant methods. This can be mixed and analyzed with a patient’s sample to determine if the fungus is present.

Detecting Ovarian Cancer and Risk

A UW–Madison researcher and others have developed a new ovarian cancer screening method that measures total or ionized serum calcium levels in blood samples. Women exhibiting elevated levels may be up to three times more likely to be diagnosed with ovarian cancer.

Serum calcium can be detected and quantified using standard techniques, including absorption spectrometry, ion selective electrode, fluorescent detection, etc. The measured levels can be incorporated into an overall risk score, identifying women who should undergo further CA125 and ultrasound screening.

Quantitative Comet Assay for Measuring Viral Growth and Resistance to Anti-Viral Compounds

UW-Madison researchers have developed a sensitive means of measuring viral infectivity and replication activity by monitoring flow-induced viral comet formation. A layer of host cells is contacted with a viral sample and cultured in a thin layer of liquid culture medium. Preferably, the host cells are cultured with the virus particles in a microfluidic channel. The liquid medium flows controllably through the channel to enhance the spread of the viral progeny to uninfected host cells. Infected host cells develop an observable indication of viral gene expression, like cell death. The resulting comet-like infections can be digitally imaged and computer-processed for automated quantification of the spread of viral infection.