Technologies

Research Tools : Antibodies

Technologies

Yeast-Based Intein Platform for Drug Production

UW–Madison researchers have engineered non-self-cleaving Mxe GyrA inteins shown to significantly improve the production of fusion proteins from Saccharomyces cerevisiae. The novel inteins were developed through directed evolution, and they enhance fusion protein display (up to 3x) and secretion levels (up to 30x) compared to the wild type intein. The new yeast-based platform provides a robust alternative to bacterial intein expression systems.
P150056US02

Eight Hybridoma Cell Lines Producing Monoclonal Antibodies Against La Crosse Encephalitis Virus

UW-Madison researchers have now produced eight lines of hybridomas, one of which is specific for G1, five of which are specific for G2, and two of which recognize both G1 and G2. Frozen stocks are available at UW-Madison.
P01150US

Monoclonal Antibody to Eukaryotic RNA Polymerase II

UW-Madison researchers have developed a monoclonal antibody against the largest subunit of RNA polymerase II. The antibody was isolated by using RNA polymerase II purified from wheat germ. It reacts with the largest, unproteolyzed subunit of RNA polymerase II from a variety of eukaryotic organisms. It also reacts with a synthetic peptide containing three repeats of the consensus sequence for the C-terminus heptapeptide domain.
P03157US

HERG-Specific Antisera

UW-Madison researchers have developed polyclonal IgG antibodies against 15-amino acid peptide sequences unique to HERG1. The peptide sequences were selected based on their antigenicity, surface probability and conservation among species. These sera have been validated for use in Western blot, immuno-precipitation and histological characterization of native and expressed HERG channels. In validation tests, they strongly outperformed commercially available antibodies in terms of sensitivity, selectivity and versatility for a wide range of uses.
P04019US

Novel Assay for Measuring Stress-Induced CREB Phosphorylation

UW-Madison researchers have developed a phospho-specific antibody that can be used to monitor stress-induced phosphorylation of the CREB transcription factor. CREB is phosphorylated in response to a wide variety of cell stresses that result in oxidative or radiation damage to cellular DNA. The researchers identified three sites on CREB that are phosphorylated by the ATM kinase. This affinity-purified, rabbit polyclonal antibody specifically recognizes one of these sites after phosphorylation has occurred. The antibody can be used to determine if ATM has phosphorylated CREB.
P04048US

A Polyclonal Antibody Directed Against the C-Terminus of HERG

UW-Madison researchers have developed a versatile polyclonal anti-HERG antibody. This antibody was developed in 1997 using a fusion protein that included 181 amino acids from the C-terminus of HERG. It detects HERG 1a and 1b.

The antibody’s specificity has been tested by Western blot, immunohistochemical and immunoprecipitation assays, including histological characterization of native and expressed HERG channels. In validation tests, it outperformed commercially available antibodies for a range of uses.
P05253US

Monoclonal Antibodies Specific for the Cytoplasmic Gamma-actin Isoform

UW-Madison researchers have developed five monoclonal antibodies to cytoplasmic gamma-actin. They purified full-length cytoplasmic gamma-actin from bovine brain and then co-immunized mice with the purified gamma-actin and a synthetic peptide containing 15 amino acids from a unique sequence in the N-terminus of gamma-actin. Western blots of purified actin isoforms and tissue extracts confirmed that the resulting antibodies were specific to gamma-actin, while images of muscle biopsies from a dystrophic mouse model validated the antibodies for use in immunofluorescence microscopy and as potential diagnostics in muscular dystrophy.
P05366US

Mouse Monoclonal Antibodies to the Human Nuclear Receptor, Estrogen-Related Receptor Alpha

UW-Madison researchers have developed high quality, well characterized monoclonal antibodies to ERRalpha. Because some of these antibodies bind antigen tightly but release it when eluted with a mixture of salt and a polyol, they are ideal for use in immunoaffinity chromatography.
P06105US

A Monoclonal Antibody Specific for Brain Alpha-Dystroglycan

UW-Madison researchers have developed mAb 2238E2D2, an IgG2b isotype monoclonal antibody that specifically recognizes brain alpha-dystroglycan from a variety of species. They developed this monoclonal antibody by immunizing mice with purified alpha-dystroglycan from bovine brain and screening for antibodies that reacted only with brain alpha-dystroglycan. They also created another monoclonal antibody, mAb 2237E2D1, which is specific for brain alpha-dystrogylcan from cows.
P06475US

Polyclonal Antibody That Recognizes the Sulfonylurea Receptor 1 Isoform

UW-Madison researchers have developed an antibody, known as BNJ1, that specifically recognizes SUR1. It can be used in combination with other antibodies developed by the inventors (see WARF reference numbers P08021US, P08022US, P08023US and P08024US) to distinguish SUR isoforms and splice variants.
P07041US

Mouse Monoclonal Antibodies to Epstein-Barr Virus Nuclear Antigen 1 (EBNA1)

UW-Madison researchers have produced nine extensively characterized mouse mAbs to EBNA-1, which provide new tools for studying EBV infection and EBNA-1.
P07324US

Polyclonal Antibodies Against Cardiovirus 2A, 2B, 2C, 3C, 3A, 3B and 3AB Proteins

UW-Madison researchers have developed polyclonal mouse antibodies against recombinant cardiovirus proteins. The antibodies can be used for specific molecular recognition of the cardiovirus proteins in vitro or in vivo using experimental techniques such as ELISA (enzyme linked immunosorbent assay), Western assays, protein immunoprecipitation (IP) and confocal fluorescence microscopy.
P07499US

Polyclonal Antibodies Against Equine Rhinitis A Virus 3AB, 3C and 3D Proteins

UW-Madison researchers have developed polyclonal antibodies against recombinant proteins from equine rhinitis A virus. The antibodies can be used for specific molecular recognition of the equine rhinitis A virus proteins in vitro or in vivo using experimental techniques such as ELISA (enzyme linked immunosorbent assay), Western assays, protein immunoprecipitation (IP) and confocal fluorescence microscopy.
P07500US

Mouse Monoclonal Antibody Against the Sigma70 Subunit of E. coli RNA Polymerase

UW-Madison researchers have developed a mouse monoclonal antibody (mAb) against an E. coli sigma factor. Known as 2F8, this mAb specifically recognizes the sigma70 transcription factor, which is the main sigma factor.
P07502US

Mouse Monoclonal Antibody Against the Alpha Subunit of E. coli RNA Polymerase

UW-Madison researchers have developed a mouse monoclonal antibody, known as 4RA1, which specifically recognizes the alpha subunit of E. coli RNA polymerase.
P07503US

Polyclonal Antibody That Recognizes the Sulfonylurea Receptor 2 Isoform

UW-Madison researchers have developed an antibody, known as BNJ2, that specifically recognizes SUR2. It can be used in combination with other antibodies developed by the inventors (see WARF reference numbers P07041US, P08022US, P08023US and P08024US) to distinguish SUR isoforms and splice variants.
P08021US

Polyclonal Antibody That Recognizes Both Isoforms of the Sulfonylurea Receptor

UW-Madison researchers have developed an antibody, known as BNJU, that recognizes SUR1 and SUR2. It can be used in combination with other antibodies developed by the inventors (see WARF reference numbers P07041US, P08021US, P08023US and P08024US) to distinguish SUR isoforms and splice variants.
P08022US

Polyclonal Antibody That Recognizes the 2A Variant of the Sulfonylurea Receptor

UW-Madison researchers have developed an antibody, known as BNJ39, that specifically recognizes the SUR2A splice variant. It can be used in combination with other antibodies developed by the inventors (see WARF reference numbers P07041US, P08021US, P08022US and P08024US) to distinguish SUR isoforms and splice variants.
P08023US

Polyclonal Antibody That Recognizes the 2B Variant of the Sulfonylurea Receptor

UW-Madison researchers have developed an antibody, known as BNJ40, that specifically recognizes the SUR2B splice variant. It can be used in combination with other antibodies developed by the inventors (see WARF reference numbers P07041US, P08021US, P08022US and P08023US) to distinguish SUR isoforms and splice variants.
P08024US

Monoclonal Antibodies Against Human Rhinovirus Type 16, a Model Virus for the Common Cold

A UW-Madison researcher has developed a mouse monoclonal antibody (mAb) against HRV16. This mAb, called mAb16-7, detects HRV16 and HRV1A by specifically recognizing the viral capsid protein VP2. It can be used in a variety of experimental techniques, such as immuno-staining and Western blot.
P08176US

Polyclonal Antibodies to CARM1

A UW-Madison researcher has developed polyclonal antibodies to CARM1. These antibodies were isolated from rabbit serum and have been thoroughly tested for various applications.
P08238US

Antibodies to NGAL for Detection of Cancer and Kidney Damage

UW-Madison researchers have developed purified antibodies to NGAL. These unique antibodies can be used in immunoassays for the diagnosis and prognosis of some types of cancer, renal failure and other diseases.
 
P09185US

Phospho-Specific Antibody to Study Circadian Rhythm Gene PER2

UW–Madison researchers have developed phospho-PER2 antibodies that recognize PER2 protein phosphorylated by the enzyme casein kinase 1. The antibodies selectively recognize forms of the protein phosphorylated on Serine-662, -665 and -668.
P130048US01

hMREII Antiserum for the Detection of DNA Damage

A UW-Madison researcher has now isolated such a homolog by using a two-hybrid system to determine which proteins interact with the repair enzyme, DNA ligase I. The gene, termed hMREII, maps to human chromosome 11q21 in a region frequently associated with cancer-related chromosomal abnormalities.

The researcher cloned a 1900 base pair portion of the hMREII gene into a bacterial expression vector and used the bacterially produced and purified hMREII to immunize rabbits. The resulting antiserum detects the hMre11 protein, which has been shown to associate with radiation-induced double stranded DNA breaks, as well as human telomeres. Congenitally acquired defects in hMre11 and its binding partner, Nbs1, result in cancer predisposition and ionizing radiation sensitivity; in these diseases, the localization of hMre11 to DNA breaks is compromised. Thus, the antiserum is useful for detecting DNA damage and assessing the functional status of hMre11 and its associated proteins. The Mre11 complex, consisting of these proteins, is a central mediator of DNA damage responses in all eukaryotic cells.
P95198US

Bone Morphogenetic Protein-1 Biological Materials

UW-Madison researchers have developed DNA clones, antibodies, vectors and cell lines for expressing bone morphogenetic protein-1 (BMP-1). These materials can be used to produce recombinant BMP-1.
P96200US

Monoclonal Antibodies That Recognize Recombinant Mengovirus 3C Proteinase

UW-Madison researchers have created hybridoma cell lines producing monoclonal antibodies raised to recombinant Mengo 3C protease; these antibodies are designated 6D10, 8F10 and 10C6. This material may be used in the specific molecular recognition and detection of protein 3C (protease), which is encoded within the genome of Mengovirus of the family Picornaviradae.
P97028US

Monoclonal and Polyclonal Antibodies Raised to Recombinant Mengovirus 3D Polymerase

UW-Madison researchers have created polyclonal antibodies and various hybridoma cell lines producing monoclonal antibodies raised to recombinant Mengo 3D polymerase. These antibodies are designated 1B6, 3C5, 10D3, 2F11, 3C6, 2F6, 3B7, 10B3, 1D6, 5F6, and 8D10. This material can be used in the specific molecular recognition and detection of the protein 3D polymerase that is encoded within the genome of Mengovirus in the family Picornavirae.
P97029US