Wisconsin Alumni Research Foundation

Research Tools
Research Tools
Monoclonal Antibody to Eukaryotic RNA Polymerase II
WARF: P03157US

Inventors: Richard Burgess, Nancy Thompson

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in a monoclonal antibody against the largest subunit of RNA polymerase II.
Overview
The enzyme RNA polymerase II synthesizes precursors to mRNAs and small nuclear RNAs. Sequence analysis of the largest subunit of RNA polymerase II has revealed an unusual heptapeptide repeat at the molecule’s C-terminal end. This domain is highly conserved in mammals and yeast, and less well conserved in Drosophila.
The Invention
UW-Madison researchers have developed a monoclonal antibody against the largest subunit of RNA polymerase II. The antibody was isolated by using RNA polymerase II purified from wheat germ. It reacts with the largest, unproteolyzed subunit of RNA polymerase II from a variety of eukaryotic organisms. It also reacts with a synthetic peptide containing three repeats of the consensus sequence for the C-terminus heptapeptide domain.
Applications
  • Studying the role of the heptapeptide repeat region in transcription initiation
  • Studying RNA polymerase II in various organisms
Key Benefits
  • Applicable to a variety of eukaryotic organisms
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854

WARF