Through Technologies

WARF: P96014US

Primate Embryonic Stem Cells


James Thomson

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in a purified preparation of primate embryonic stem (ES) cells.
OVERVIEWA UW-Madison researcher has developed a purified preparation of primate embryonic stem (ES) cells isolated from two primate species: the common marmoset (Callithrix jacchus) and the rhesus monkey (Macaca mulatta). These cell lines are true pluripotent ES lines. That is, they proliferate indefinitely in an undifferentiated state in vitro, and then can differentiate to cells derived from all three embryonic germ layers (ectoderm, mesoderm and endoderm), even after prolonged culture. The primate ES cell lines are characterized by the following cell surface markers: SSEA-1 (-), SSEA-3 (+), SSEA-4 (+), TRA-1-60 (+), TRA-1-81 (+) and alkaline phosphatase (+). Methods for isolating primate ES cell lines are also provided.
  • Drug discovery
  • Tissue transplantation
  • Disease research
  • Capable of prolonged (greater than one year) proliferation in an undifferentiated state
  • Can differentiate into any cell, including bone, cartilage, muscle, neural and hematopoetic cells, derived from the three embryonic germ layers 
  • Maintain a normal karyotype (e.g. remain euploid) during long-term culture
  • Retain ability to form trophoblast in vitro and express chorionic gonadotropin 
  • Allow the creation of transgenic primate tissue or animal models for the study of human genetic diseases
  • May be induced to differentiate to specific cell types for tissue transplants to treat human diseases such as Parkinson’s (dopanergic neurons), juvenile onset diabetes (pancreatic beta-islet cells) and AIDS (lymphocytes)
  • Techniques may be used to derive ES cells from other primate species
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