Wisconsin Alumni Research Foundation

Technology

Assembly of Full-Length Genes from DNA Arrays

The ability to chemically synthesize single-stranded oligonucleotides has had a profound impact on research and medicine. Yet existing strategies have their limitations. One critical limitation is the...
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Lloyd Smith, Cheng-Hsien Wu | P120014US02

Technology

Cleaving Double-Stranded DNA at User-Chosen Sites

Restriction enzymes are used widely to cleave double-stranded DNA. However, a given restriction enzyme can only cleave a DNA molecule at the specific nucleotide sequence that corresponds to the recogn...
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Michael Cox, Marielle Eichhorn-Gruenig, James Keck | P100286US02

Technology

Markerless Gene Replacement Plasmids for E. coli

Microbial genome sequencing projects uncover large numbers of new genes. Functional analyses of these genes require targeted modifications of particular DNA sequences in their chromosomal locations us...
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Frederick Blattner, Gyorgy Posfai, Vitaliy Kolisnychenko, Zsuzsa Bereczki | P01153US

Technology

Tn5 Transposase Showing Enhanced Activity

The low mobility of bacterial transposons, such as Tn5, makes it difficult for researchers to detail the molecular transposition process and to exploit transposition for uses such as the development o...
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William Reznikoff, Mindy Steiniger-White, Jeremy Metzler | P02358US

Technology

Promoter-Trap Plasmid for Identifying Promoters

Promoters are genetic regulatory elements that drive gene expression in cells under certain conditions. One way to identify promoters is to use a promoter-trap vector, which is a plasmid containing a ...
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Jo Handelsman, Anne Katherine Dunn | P03159US

Technology

Mutated Tn5 Transposase Proteins and Their Uses

The low mobility of bacterial transposons, such as Tn5, makes it difficult for researchers to detail the molecular transposition process and to exploit transposition for uses such as the development o...
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William Reznikoff, Richard Gradman | P03381US

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