UW-Madison researchers have developed a titin DNA construct (a ‘titin minigene plasmid’) that is useful for identifying agents that modulate the titin size reduction that correlates with a...
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Coronaviruses are a group of RNA viruses that can cause disease. Some, such as the common cold, can cause mild disease, while others, such as COVID-19, can be lethal. An unusual characteristic of coro...
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Disclosed is a method of isolating, purifying, or concentrating a target compound. The method includes the steps of onjugating the target compound to an epitope for a polyol-responsive monoclonal anti...
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R1 and R2 are hydrogen or C1 to C12 linear or branched alkyl; R3 is hydrogen, C1 to C12 linear or branched alkyl, or nitrogen protecting groups; X is oxygen or nitrogen; and when X is oxygen, R4 is ab...
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Compounds of Formula I: [Image Omitted] wherein: R1 and R1′ are independently selected from hydrogen, halogen, C1-to-C12 linear or branched hydroxyalkyl, C1-to-C12 linear or branched halo-alkyl, ...
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The present disclosure provides systems and methods for characterizing the interaction of free radicals with various materials and the use of known interactions to isolate free radical generation from...
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Chemoselective transformations are of key importance in modern chemical biology. Protein esterification can, for example, be employed for labeling, enhanced cellular uptake and other useful protein mo...
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The utility of many biologic drugs is limited by inefficient cellular delivery. There is a particular need for methods and reagents that facilitate delivery of peptides and proteins into the interior ...
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Fluorescent proteins can be found today in most molecular biology laboratories. Since the discovery of Green Fluorescent Protein (GFP) in the early 1960s, fluorescent molecules have revolutionized man...
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Biomarker engineering and development has evolved to answer infinitely complex biological questions and the discovery of novel molecules could provide additional tools for biotechnology. Fluorescent r...
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Disulfide bonds between cysteine residues are the most common crosslinking agents in proteins. Reducing these bonds is an ordinary procedure in biochemistry and biotechnology. The most commonly used r...
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Most biological processes involve multiple proteins interacting with each other. While the principles governing protein-protein interactions are not fully understood, it is known that a small subset o...
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Restriction enzymes are commonly used to cleave double-stranded DNA. These enzymes bind to specific sequences of DNA (the ‘restriction site’) and cleave the DNA either at the recognition site or a...
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Membrane proteins perform many crucial functions in vivo but are difficult to manipulate and study because they are not soluble in simple aqueous buffers. Solubilizing membrane proteins for physical c...
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Disulfide bonds between cysteine residues are the most common crosslinking agents in proteins. Reducing these bonds is an ordinary procedure in biochemistry and biotechnology. The most commonly used r...
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Integral membrane proteins are crucial cellular components, helping to transfer material and signals through the cytoplasm or between different cell compartments. Analyzing the structures and function...
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Membrane proteins play crucial roles in biology but are difficult to handle and analyze because of their physical properties. The structures of these proteins display extensive nonpolar surfaces, whic...
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The ability of bacteria to adapt and resist antibiotics has led to growing initiatives in the United States and other countries to develop new drugs and modify existing chemical structures to increase...
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Proteins, polysaccharides, biofilms and other contaminants may collect on surfaces over time in a process known as surface fouling. The identification of agents that prevent the adsorption of biofouli...
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The pathogen Mycobacterium tuberculosis causes tuberculosis, one of the world’s deadliest diseases.  Because the desaturase DesA3 is essential for survival of M. tuberculosis, this enzyme i...
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Identification and characterization of promising target molecules often presents a bottleneck in the search for new drugs. A variety of high throughput methods for probing protein interactions have em...
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The yeast two-hybrid system is a powerful technique used to detect protein-protein interactions. The technique has been used to establish physical interactions between genetically identified proteins,...
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Membrane proteins perform many crucial functions in vivo.  Physical characterization of these proteins, including the determination of three-dimensional crystal structures, could improve fundamen...
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Three hybrid screens are used to detect RNA-protein interactions.  A problem with three hybrid screens has been high background due to some cDNAs binding and activating transcription in the absen...
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Biological membranes, such as cell membranes, are intrinsically impermeable to ions and polar molecules. Thus, transport of ions through biological membranes requires special channel or pump proteins ...
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