Technologies
PDF


WARF: P150031US02

Enhancing Cell Penetration to Improve Drug Delivery


INVENTORS -

Ronald Raines, Kristen Andersen

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing methods to enhance cellular uptake of fluorenylated proteins, peptides and other cargo molecules.
OVERVIEWThe utility of many biologic drugs is limited by inefficient cellular delivery. There is a particular need for methods and reagents that facilitate delivery of peptides and proteins into the interior fluid (cytosol) of cells. The vast majority (>90 percent) of biologics that enter a cell’s endosomes fail to reach the cytosol, where they can be of greater value.

Until now, there have been few methods for modifying a peptide or protein (that does not naturally enter a cell) to enhance its uptake into the cytosol in a bioreversible manner. Being able to do so has implications for biological research as well as the clinic. For example, dysfunctional proteins could be replaced with functional ones, and misbehaving proteins could be antagonized with specific antibodies.
THE INVENTIONUW–Madison researchers have developed a method for enhancing cellular uptake of a cargo molecule by covalently bonding fluorenyl groups to it. The fluorenylated molecule is then contacted with the cell or tissue. Cellular uptake may be in vivo or in vitro and includes at least partial penetration into the cytosol.
APPLICATIONS
  • Enhanced cellular delivery/penetration of drugs, proteins, nucleic acids and other molecules
KEY BENEFITS
  • First bioreversible method of its kind
  • Increased cytosolic uptake
STAGE OF DEVELOPMENTThe method has been used to deliver GFP to the cytosol. GFP is an anionic protein that has no tendency to enter mammalian cells.
Contact Information
For current licensing status, please contact Jennifer Gottwald at jennifer@warf.org or 608-960-9854.
The WARF Advantage

Since its founding in 1925 as the patenting and licensing organization for the University of Wisconsin-Madison, WARF has been working with business and industry to transform university research into products that benefit society. WARF intellectual property managers and licensing staff members are leaders in the field of university-based technology transfer. They are familiar with the intricacies of patenting, have worked with researchers in relevant disciplines, understand industries and markets, and have negotiated innovative licensing strategies to meet the individual needs of business clients.