Technologies
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WARF: P03322US

High Titer Recombinant Influenza Viruses for Vaccines and Gene Therapy


INVENTORS -

Yoshihiro Kawaoka

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing an efficient technique and system for producing high titer influenza A virus in vertebrate cells in the absence of helper virus.
OVERVIEWInfluenza is a major disease in humans that can be prevented by vaccination. Because new epidemic strains arise every year or two, the conventional influenza vaccine must be adapted almost every year, making efficient vaccine production a major objective of pharmaceutical companies.
THE INVENTIONA UW-Madison researcher has developed an efficient technique and system for producing high titer influenza A virus in vertebrate cells in the absence of helper virus. The technology takes advantage of a reverse genetics system created by Dr. Kawaoka that allows efficient production of influenza virus for vaccines and gene therapy applications (see WARF reference number P03252US). In the technology described here, the inventor developed a new set of plasmids for use with the reverse genetics system. The plasmids contain cDNAs from a high titer influenza virus isolate; the promoter for RNA polymerase I or RNA polymerase II; and the terminator sequence for RNA polymerase I. When these constructs are transfected into host cells, the cells consistently generate high yields of infectious influenza particles.
APPLICATIONS
  • Influenza vaccine production
  • Rapid production of attenuated live-virus vaccine during a suspected pandemic
  • Vaccine vectors for gene therapy
  • Viral mutagenesis studies
KEY BENEFITS
  • Greatly enhances influenza viruses as vaccine vectors
  • Viruses can be engineered to express foreign proteins or immunogenic epitopes.
Contact Information
For current licensing status, please contact Jennifer Gottwald at jennifer@warf.org or 608-960-9854.
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Since its founding as a private, nonprofit affiliate of the University of Wisconsin–Madison, WARF has provided patent and licensing services to UW–Madison and worked with commercial partners to transform university research into products that benefit society. WARF intellectual property managers and licensing staff members are leaders in the field of university-based technology transfer. They are familiar with the intricacies of patenting, have worked with researchers in relevant disciplines, understand industries and markets, and have negotiated innovative licensing strategies to meet the individual needs of business clients.

The University of Wisconsin and WARF –
A Single Location to Accelerate Translational Development of New Drugs

UW–Madison has the integrative capabilities to complete many key components of the drug development cycle, from discovery through clinical trials. As one of the top research universities in the world, and one of the two best-funded universities for research in the country, UW–Madison offers state-of-the-art facilities unmatched by most public universities.

These include the Small Molecule Screening Facility at the UW Comprehensive Cancer Center; the Zeeh Pharmaceutical Experiment Station, which provides consulting and laboratory services for developing formulations and studying solubility, stability and more; the Waisman Clinical Biomanufacturing Facility; the Wisconsin Institute for Medical Research, which provides UW–Madison with a complete translational research facility; and the innovative, interdisciplinary Wisconsin Institutes for Discovery, home to the private, nonprofit Morgridge Institute for Research and its public twin, WID, part of the university's graduate school. The highly qualified experts at these facilities are ready to work with you to create a library of candidates for drug development.