WARF: P99275US

Serum-Free Cultivation of Primate Embryonic Stem Cells


James Thomson

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing methods for stably culturing primate embryonic stem cells in serum-free media.
OVERVIEWAll known media for the stable cultivation of embryonic stem cells (ES cells) require serum; however, batches of fetal bovine serum vary dramatically in their ability to support ES cell growth. Cloning efficiency is approximately ten-fold higher in serum-free media, but cells in these media differentiate in seven to ten days.
THE INVENTIONA UW-Madison researcher has developed methods for stably culturing primate embryonic stem cells in serum-free media. The researcher discovered that adding basic fibroblast growth factor (bFGF) to serum-free media allows undifferentiated ES cells to proliferate in culture for long periods of time.
  • Culturing primate ES cells
  • Allows stable proliferation for over one year
  • Cells maintain ability to differentiate into all cell types.
  • Supports clonal derivation at a higher efficiency than serum-containing media
  • No animal serum required
For More Information About the Inventors
Contact Information
For current licensing status, please contact Andy DeTienne at or 608-960-9857.
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