WARF: P01323US

  • Assigned to WARF as biological material.

Transgenic Mice Expressing Human Alkaline Phosphatase


Eric Sandgren, William Kisseberth, Jan Lohse, Phil Soriano

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing transgenic mice that ubiquitously express the marker gene hPAP.
OVERVIEWThe ability to unambiguously mark a cell’s genotype is essential for studies in which genetically distinct cell populations must be distinguished from one another in vivo.
THE INVENTIONUW-Madison researchers have now developed transgenic mice that ubiquitously express the marker gene human placental alkaline phosphatase (hPAP). They used an 800-base pair fragment of a promoter region from ROSA26 cells in a genetic construct with hPAP.

Several lines of transgenic mice from both the FVB/N and C57BL/6 mouse strains were created using the microinjection technique. The ROSA26 promoter directs ubiquitous expression of hPAP during embryonic and postnatal development in the mice.
  • Marking donor cells in transplantation studies
  • Embryonic chimera studies and lineage analyses
  • Marker gene is expressed ubiquitously.
  • Marker is easily detectable with no background staining.
  • Permits monitoring of engraftment of transplanted cells
  • Because hPAP is heat stable, fixed- and paraffin-embedded tissue sections can be incubated directly with substrate.
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Contact Information
For current licensing status, please contact Jennifer Gottwald at or 608-960-9854.
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