Technologies
PDF


WARF: P01323US

  • Assigned to WARF as biological material.

Transgenic Mice Expressing Human Alkaline Phosphatase


INVENTORS -

Eric Sandgren, William Kisseberth, Jan Lohse, Phil Soriano

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing transgenic mice that ubiquitously express the marker gene hPAP.
OVERVIEWThe ability to unambiguously mark a cell’s genotype is essential for studies in which genetically distinct cell populations must be distinguished from one another in vivo.
THE INVENTIONUW-Madison researchers have now developed transgenic mice that ubiquitously express the marker gene human placental alkaline phosphatase (hPAP). They used an 800-base pair fragment of a promoter region from ROSA26 cells in a genetic construct with hPAP.

Several lines of transgenic mice from both the FVB/N and C57BL/6 mouse strains were created using the microinjection technique. The ROSA26 promoter directs ubiquitous expression of hPAP during embryonic and postnatal development in the mice.
APPLICATIONS
  • Marking donor cells in transplantation studies
  • Embryonic chimera studies and lineage analyses
KEY BENEFITS
  • Marker gene is expressed ubiquitously.
  • Marker is easily detectable with no background staining.
  • Permits monitoring of engraftment of transplanted cells
  • Because hPAP is heat stable, fixed- and paraffin-embedded tissue sections can be incubated directly with substrate.
ADDITIONAL INFORMATION
For More Information About the Inventors
Contact Information
For current licensing status, please contact Jennifer Gottwald at jennifer@warf.org or 608-960-9854.
The WARF Advantage

Since its founding in 1925 as the patenting and licensing organization for the University of Wisconsin-Madison, WARF has been working with business and industry to transform university research into products that benefit society. WARF intellectual property managers and licensing staff members are leaders in the field of university-based technology transfer. They are familiar with the intricacies of patenting, have worked with researchers in relevant disciplines, understand industries and markets, and have negotiated innovative licensing strategies to meet the individual needs of business clients.