Technologies
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WARF: P01324US

  • Assigned to WARF as biological material.

Transgenic Mice That Ubiquitously Express Enhanced Green Fluorescent Protein


INVENTORS -

Eric Sandgren, William Kisseberth, Jan Lohse, Phil Soriano

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing transgenic mice that ubiquitously express the marker gene EGFP.
OVERVIEWThe ability to unambiguously mark a cell’s genotype is essential for studies in which genetically distinct cell populations must be distinguished from one another in vivo.
THE INVENTIONUW-Madison researchers have developed transgenic mice that ubiquitously express the marker gene enhanced green fluorescent protein (EGFP). They used an 800-base pair fragment of a promoter region from ROSA26 cells in a genetic construct with EGFP.

Several lines of transgenic mice were created using the microinjection technique. The ROSA26 promoter directs ubiquitous expression of EGFP during embryonic and postnatal development in the mice.
APPLICATIONS
  • Marking donor cells in transplantation studies
  • Marking genotypes in pre-implantation embryos
  • Embryonic chimera studies and lineage analyses
  • Experiments that require genetic marking of subpopulations of cells within a larger unmarked cell population
KEY BENEFITS
  • Marker gene is expressed ubiquitously.
  • Marker is easily detectable with no background staining.
  • Permits monitoring of engraftment of transplanted cells
  • GFP can be visualized in living cells.
ADDITIONAL INFORMATION
For More Information About the Inventors
Contact Information
For current licensing status, please contact Jennifer Gottwald at jennifer@warf.org or 608-960-9854.
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