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WARF: P05366US

  • Assigned to WARF as biological material.

Monoclonal Antibodies Specific for the Cytoplasmic Gamma-actin Isoform


INVENTORS -

James Ervasti, Laurin Hanft

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing five monoclonal antibodies to cytoplasmic gamma-actin.
OVERVIEWThe six mammalian actin genes encode a highly conserved family of cytoskeletal protein isoforms important in cell structure, mechanical stability, and motility. Actin isoforms exhibit tissue-specific expression and distinct subcellular distributions, suggesting that each isoform may serve a different function. Although commercially available monoclonal antibodies exist for most isoforms of actin, the only available antibodies specific for the gamma-cytoplasmic isoform were extremely variable polyclonal antibodies.
THE INVENTIONUW-Madison researchers have developed five monoclonal antibodies to cytoplasmic gamma-actin. They purified full-length cytoplasmic gamma-actin from bovine brain and then co-immunized mice with the purified gamma-actin and a synthetic peptide containing 15 amino acids from a unique sequence in the N-terminus of gamma-actin. Western blots of purified actin isoforms and tissue extracts confirmed that the resulting antibodies were specific to gamma-actin, while images of muscle biopsies from a dystrophic mouse model validated the antibodies for use in immunofluorescence microscopy and as potential diagnostics in muscular dystrophy.
APPLICATIONS
  • Potentially useful in the diagnosis of muscular dystrophy
KEY BENEFITS
  • Provides—for the first time—monoclonal antibodies to the gamma-cytoplasmic isoform of actin
  • Effective research tools
ADDITIONAL INFORMATION
Contact Information
For current licensing status, please contact Jennifer Gottwald at jennifer@warf.org or 608-960-9854.
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