WARF: P94062US

  • Assigned to WARF as biological material.

Fusion Protein Expression Vectors, CMV-GST and CMV-tac-GST


William Fahl, T. Herbert Manoharan, Jean Gallo, Andrew Gulick

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in two plasmid vectors that allow expression of the GST pi protein.
OVERVIEWEnzymes of the glutathione S-transferase (GST) family are present in both eukaryotes and prokaryotes. As much as 10 percent of the cytosolic protein in some mammalian organs can consist of GSTs.
THE INVENTIONUW-Madison researchers have developed two plasmid vectors that allow expression of the GST pi protein. These vectors were tested in bacterial and mammalian hosts, where they allowed expression of the GST protein as expected. Pieces of DNA can be inserted into a cloning site in these vectors. The encoded recombinant gene then can be expressed in bacterial and mammalian cells, where it will direct the synthesis of a fusion protein in which the amino terminal portion is a GST and the carboxy terminal end is the protein of interest.
  • Useful to create a fusion protein with GST and another protein
  • This material is available through the American Type Culture Collection ( #87629
For More Information About the Inventors
Contact Information
For current licensing status, please contact Rafael Diaz at or 608-960-9847.
The WARF Advantage

Since its founding in 1925 as the patenting and licensing organization for the University of Wisconsin-Madison, WARF has been working with business and industry to transform university research into products that benefit society. WARF intellectual property managers and licensing staff members are leaders in the field of university-based technology transfer. They are familiar with the intricacies of patenting, have worked with researchers in relevant disciplines, understand industries and markets, and have negotiated innovative licensing strategies to meet the individual needs of business clients.