Technologies
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WARF: P05096US

Nuclease Inhibitors and Methods for Their Use


INVENTORS -

Ronald Raines, Bryan Smith, David Lynn, Matthew Soellner

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing polymers that inhibit nucleases, particularly ribonucleases.
OVERVIEWBiochemical research and biotechnology rely on nucleic acids; however, nucleic acids frequently encounter nucleases that can negatively affect experiments. Although nuclease inhibitors are often used in vitro to mitigate damage to RNA and other nucleic acids, existing inhibitors suffer from several drawbacks.
THE INVENTIONUW-Madison researchers have identified a class of anionic oligomers and polymers that inhibit nucleases, particularly ribonucleases. The inhibitors are polymers of vinyl sulfonate. When nucleases are brought into contact with them in vitro, the polymers are capable of reversibly inhibiting or inactivating the nucleases. The researchers also have developed a method for purifying commercial buffers of these inhibitors.
APPLICATIONS
  • Removing undesired ribonucleases and deoxyribonucleases, as well as nucleic acid-binding proteins, from biological media
KEY BENEFITS
  • Can be attached to surfaces for removal of nucleases from media, solutions, or other liquids in contact with the solid surface
  • Extremely stable and simple to prepare
ADDITIONAL INFORMATION
For More Information About the Inventors
Related Intellectual Property
Contact Information
For current licensing status, please contact Jennifer Gottwald at jennifer@warf.org or 608-960-9854.
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Since its founding in 1925 as the patenting and licensing organization for the University of Wisconsin-Madison, WARF has been working with business and industry to transform university research into products that benefit society. WARF intellectual property managers and licensing staff members are leaders in the field of university-based technology transfer. They are familiar with the intricacies of patenting, have worked with researchers in relevant disciplines, understand industries and markets, and have negotiated innovative licensing strategies to meet the individual needs of business clients.