Wisconsin Alumni Research Foundation

Research Tools
Research Tools
Faster, Cheaper DNA Synthesis
WARF: P04400US02

Inventors: Michael Sussman, Kathryn Richmond, Matt Rodesch

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing a method for creating hundreds of thousands of oligonucleotides at dramatically lower cost.
Overview
Synthesizing DNA and genes is an important tool in biotechnology. It has become commonplace to synthesize short DNA chains, called oligonucleotides, on a substrate and use these as building blocks to assemble longer DNA sequences.

While the process can be automated, synthesizing long genes remains expensive. Also, current methods produce extremely small amounts of DNA, which makes physical handling and dilution a challenge.
The Invention
UW–Madison researchers have developed a high yield method for synthesizing oligonucleotides with defined sequences and soluble in solution.

In the new method, DNA starting material is synthesized in parallel on a microarray, amplified via polymerase chain reaction (PCR) and cleaved. The cleaved fragments then can be used to assemble a double-stranded DNA target sequence.

Hundreds of thousands of oligonucleotides can be synthesized and assembled into many individual genes by this process.
Applications
  • Oligonucleotide production
  • Gene synthesis and site-directed mutagenesis
Key Benefits
  • High yield
  • Fast and efficient
  • Inexpensive – oligonucleotides produced for pennies on the dollar
Additional Information
For More Information About the Inventors
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854

WARF