Wisconsin Alumni Research Foundation

Research Tools
Research Tools
DNA Synthesis with Error Reduction
WARF: P05071US

Inventors: Peter Belshaw, Michael Sussman, Franco Cerrina, James Kaysen, Brock Binkowski, Kathryn Richmond

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing a microarray technique for making DNA constructs of virtually any size with minimal error.
Overview
Synthesizing DNA is an important part of biotechnology but current methods are useful only for relatively short gene sequences. Researchers are forced to construct larger genes through time-consuming assembly of short segments called oligonucleotides.

Microarray technology has sped up the process. However, newly synthesized sequences will invariably contain mismatches due to chemical side reactions, resulting in sequence errors. The problem only gets worse when synthesizing long DNA strands. Thus, it is critical to eliminate the DNA molecules containing errors.
The Invention
UW–Madison researchers have developed a method to reduce errors in synthesized DNA and therefore produce genes of correct sequence.

In the new process (termed ‘consensus shuffling’), DNA sequences are either assembled or digested into shorter fragment and then permitted to hybridize. The double-stranded fragments are exposed to a selective DNA filtering agent to remove mismatched sequences from the population. Finally, the surviving fragments are used as templates for PCR amplification to produce full-length sequences.
Applications
  • DNA synthesis
Key Benefits
  • May reduce the number of oligonucleotides with errors by several orders of magnitude
  • Frees researchers from relying on commercially available vectors
  • Saves time and cost
  • Method is especially suited for longer genes.
Stage of Development
The researchers have demonstrated a fourfold reduction in sequence errors.
Additional Information
For More Information About the Inventors
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854

WARF