System And Method For Presenting Large Dna Molecules For Analysis
Inventors: David Schwartz, Kristy Kounovsky-Shafer, Juan Hernandez-Ortiz, Theo Odijk, Juan DePablo, Kyubong Jo
Systems and methods for presenting nucleic acid molecules for analysis are provided. The nucleic acid molecules have a central portion that is contained within a nanoslit. The nanoslit contains an ionic buffer. The nucleic acid molecule has a contour length that is greater than a nanoslit length of the nanoslit. An ionic strength of the ionic buffer and electrostatic or hydrodynamic properties of the nanoslit and the nucleic acid molecule combining to provide a summed Debye length that is greater than or equal to the smallest physical dimension of the nanoslit.