Technologies

Veterinary : Companion animals

Technologies

Diagnostic Kit for Blastomycosis

UW–Madison researchers have developed a method for obtaining highly pure native BAD-1 protein that could be used to detect B. dermatitidis infection.

A solution containing native BAD-1 protein or fragments is collected from cultured fungus strains. The solution is combined with nickel-chelating resin, washed and eluted to obtain a highly pure form without the need for more expensive recombinant methods. This can be mixed and analyzed with a patient’s sample to determine if the fungus is present.
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Early Detection of Hemangiosarcoma and Angiosarcoma

UW-Madison researchers have developed a simple, sensitive and specific test for detecting hemangiosarcoma in its early stages in dogs at risk. The inventors discovered that the “primitive” endothelial cells associated with hemangiosarcoma or angiosarcoma, a similar tumor that affects humans, express a specific combination of proteins on their surface. To diagnose hemangiosarcoma or angiosarcoma, multiparameter flow cytometry can be used to detect cells that express these proteins.
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H3 Equine Influenza A Virus

UW-Madison researchers have developed an isolated H3 equine influenza A virus, as well as methods of preparing and using the virus. This virus, which was isolated following a May 2003 outbreak of respiratory disease in horses, represents a new line of equine influenza virus. It is genetically related to a virus that caused an outbreak of influenza among horses in South Africa and to a virus isolated from greyhound dogs in Florida. This virus will be useful as part of an up-to-date vaccine against equine influenza.
P05092US

Method to Diagnose and Treat Degenerative Joint Disease

UW-Madison researchers have developed methods for detecting and treating joint disease, particularly in joints containing a functionally important intra-articular ligament or tendon. To diagnose joint or ligament degeneration, a sample is taken from a joint or ligament and tested for the expression of cathepsin K, cathepsin S, or tartrate-resistant acid phosphatase (TRAP). Because these enzymes are up-regulated during the development of inflammatory arthritis or ligament degeneration, increased levels indicate the presence of disease. Treatment of joint or ligament disease would involve inhibiting the activity of cathepsin K, cathepsin S, or TRAP.
P03092US

Method to Assess Knee Stability in Dogs

UW-Madison researchers have developed a device that measures the stability of the canine knee, providing information needed to diagnose damage to the cranial cruciate ligament. Specifically, the new device is a platform with two moving pieces. The dog's femur (top portion of the leg) is strapped to one of the pieces and the tibia (lower portion) is strapped to the other piece. An X-ray image is taken while a small force is applied to the tibia, and then a second radiograph is acquired as the force is applied in the opposite direction. Next, the two images are superimposed to measure the total joint translation. By looking at these images, veterinarians can diagnose intact, partially ruptured and completely ruptured cruciate ligaments.
P03255US

Assay for Propensity for Canine Malignant Hyperthermia

UW–Madison researchers have identified the mutation that causes MH in dogs and developed a method of determining whether a canine is susceptible to malignant hyperthermia. To determine if a dog is susceptible, a nucleic acid sample is obtained and examined for the presence or absence of a T1640C mutation. If the mutation is present, the canine is susceptible to MH.
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Method to Prevent Formation and Enhance Breakdown of Bezoars

UW-Madison researchers have developed methods for preventing gastrointestinal bezoar formation and reducing the size of gastrointestinal bezoars in humans and non-human animals. The methods use a food-grade fat emulsifier to reduce the size of a bezoar, permitting it to pass from the animal’s digestive system. An exogenously administered protease may be used in combination with the emulsifier.
P00324US

Recombinant, Attenuated Vaccine for Blastomyces Dermatitidis to Prevent Fungal Infection

UW-Madison researchers have successfully engineered an attenuated, replication-competent strain of B. dermatitidis that is incapable of expressing the cell wall protein WI-1. This recombinant, attenuated strain serves as a highly effective vaccine against the systemic, lethal infectious disease caused by B. dermatitidis and possibly against related fungi. In vitro, the number of lung colony forming units, as a sign of lung infection, revealed a substantial decrease in wild type infection.

The present invention describes use of the genetically engineered fungus as a vaccine given subcutaneously in multiple doses. Dosages can be administered at high levels to provide infection resistance yet low enough so that the fungus is cleared from the body. The researchers are currently conducting in vivo studies with dogs.
P00200US

Ringworm Fungal Strain for Infection Studies

UW–Madison researchers have established that a strain of M. canis, called UW-8, is particularly useful in experimental infection studies. The strain produces a uniquely bright and consistent fluorescence that makes ringworm lesions much easier to identify and measure.

The researchers have used UW-8 for infection studies since 1993, and the attributes of the strain are supported by a body of peer-reviewed literature. UW-8 was selected from natural field strains and can be cultured readily.
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Isolated Canine Influenza H3N2 Virus for Vaccine Production

Researchers from the Wisconsin Veterinary Diagnostic Laboratory have obtained and identified isolated H3N2 influenza virus from canines that tested positive for influenza antigen during the 2015 epidemic. The virus has been amplified in eggs as well as cell culture (MDCK cells) for possible use in vaccine production.

These isolates were not previously shown to be circulating in the United States prior to 2015. They were originally identified from a canine nasal swab as influenza A at the WVDL, and the sample was then typed as an N2 strain using an in-house real-time PCR assay.
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Isolated Influenza H3N2 Virus for Potential Feline Vaccine

Researchers from the Wisconsin Veterinary Diagnostic Laboratory have isolated H3N2 influenza virus from felines infected during the 2016 outbreak. Characterization and sequencing efforts are ongoing.
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