UW-Madison researchers have developed an improved covalent labeling system for use in protein footprinting. The innovation of the present disclosure advances a currently existing technology -- a coval...
Learn More

Metagenomics is the study of genetic material from environmental samples for detecting microbes, especially pathogens. It is increasingly used within clinical and industrial settings. While metagenomi...
Learn More

UW-Madison researchers have developed a set of novel isobaric chemical tags, DiLeu-Biotin-Azide (DBA), that could be useful in proteomics, particularly quantification of post translational modificatio...
Learn More

UW-Madison researchers have developed and validated a novel “parasitome” method to accurately characterize parasites in samples. Like published metabarcoding protocols, the new method consists of ...
Learn More

UW-Madison researchers have developed photo-cleavable anionic surfactants, particularly 4-hexylphenylazosulfonate (Azo) and sodium 4-hexylphenylazosulfonate derivatives, which can be rapidly degraded ...
Learn More

UW-Madison researchers developed an improved method of gene editing human iPSCs through CRISPR-Cas. They added the histone deacetylase small molecule inhibitor Trichostatin A (TSA) before addition of ...
Learn More

Lactic acid bacteria constitute a large group of Gram-positive bacteria (over 200 species described), of which Lactobacillus is the largest genus in the lactic acid bacteria group. Members of the Lact...
Learn More

Differentiation of normal stem cells into the correct cell lineage is a tightly controlled process that involves cell-cell and cell-extracellular matrix interactions as well as signaling through cellu...
Learn More

A molecule sequencer uses a planar nanochannel for aligning molecules to flow past impedance interrogation electrodes mounted across the channel for rapid sequencing. The electrodes may be fixed to th...
Learn More

Systems and methods for protein engineering. The systems include a sequence testing subsystem and a machine learning subsystem. The sequence testing subsystem is configured to express proteins and tes...
Learn More

Metabolomics, or the systematic study of all metabolites in an organism, is one of the fastest growing areas of scientific research. Nuclear magnetic resonance (NMR) spectroscopy is one tool used to a...
Learn More

‘Nanopore sequencing’ allows a single molecule such as DNA to be analyzed without conventional tools like chemical labels or costly optical instruments. In the process, DNA strands and a stream of...
Learn More

Humans are diploid organisms whose (non-sex) cells contain two copies of each chromosome – one from each parent. Each of these two copies may be distinguished by genetic variations such as insertion...
Learn More

Proteomics is a relatively new discipline that is a large scale study of proteins. It includes systematically identifying the sequence, characterizing the structure and evaluating the function of all ...
Learn More

Protein characterization has become an integral part of modern biology, even inspiring a new discipline known as proteomics, or the classification of proteins based on the genome of an organism.  MS ...
Learn More

Tandem mass spectrometry (MS/MS) is a technique capable of identifying large numbers of proteins in complex biological samples. In the process, peptides are ionized and an initial survey scan (i.e., a...
Learn More

‘Nanopore sequencing’ holds the potential for sequencing a single molecule of DNA without the need for conventional tools like chemical labels or costly optical instruments. This promising method ...
Learn More

Mass spectrometry (MS) is a vital tool for identifying and tallying the different proteins, peptides and other molecules in a sample. To determine relative abundance, reagent tags are used to label th...
Learn More

Antibodies are used widely in biological and medical research to bind to and help isolate molecules of interest.  However, the reduced stability and production yields of antibody fragments frequently...
Learn More

As resources shift from genome sequencing to understanding and characterizing expressed genes and protein function, reliable and rapid tools for the analysis of protein composition are increasingly vi...
Learn More

Quantitative analysis in chemistry is the determination of the absolute or relative abundance of substances present in a sample. For biological samples, quantitative analysis performed via mass spectr...
Learn More

In addition to simple nucleotide polymorphisms, the human genome includes intermediate structural variations, such as amplifications, insertions, deletions, inversions and complex rearrangements. Whil...
Learn More

Microbial genome sequencing projects uncover large numbers of new genes. Functional analyses of these genes require targeted modifications of particular DNA sequences in their chromosomal locations us...
Learn More

Analysis by mass spectrometry requires that molecules first be converted to gas-phase ions. Converting small molecules into ions is straightforward; however, it was not possible to ionize large molecu...
Learn More

Microorganisms adapted to grow in exceptionally harsh environments, such as hot springs and arctic soils, are a potentially vast source of novel metabolic processes, antibiotics, enzymes and other pro...
Learn More