UW-Madison researchers have developed a phenylglyoxal-based alkyne (PGA) chemical probe exhibits a high specificity towards protein citrullination sites and offers compatibility with various azide-con...
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UW-Madison researchers have developed an improved covalent labeling system for use in protein footprinting. The innovation of the present disclosure advances a currently existing technology -- a coval...
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Metagenomics is the study of genetic material from environmental samples for detecting microbes, especially pathogens. It is increasingly used within clinical and industrial settings. While metagenomi...
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UW-Madison researchers have developed a set of novel isobaric chemical tags, DiLeu-Biotin-Azide (DBA), that could be useful in proteomics, particularly quantification of post translational modificatio...
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UW-Madison researchers have developed and validated a novel “parasitome” method to accurately characterize parasites in samples. Like published metabarcoding protocols, the new method consists of ...
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UW-Madison researchers have developed photo-cleavable anionic surfactants, particularly 4-hexylphenylazosulfonate (Azo) and sodium 4-hexylphenylazosulfonate derivatives, which can be rapidly degraded ...
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UW-Madison researchers developed an improved method of gene editing human iPSCs through CRISPR-Cas. They added the histone deacetylase small molecule inhibitor Trichostatin A (TSA) before addition of ...
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Lactic acid bacteria constitute a large group of Gram-positive bacteria (over 200 species described), of which Lactobacillus is the largest genus in the lactic acid bacteria group. Members of the Lact...
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Differentiation of normal stem cells into the correct cell lineage is a tightly controlled process that involves cell-cell and cell-extracellular matrix interactions as well as signaling through cellu...
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A molecule sequencer uses a planar nanochannel for aligning molecules to flow past impedance interrogation electrodes mounted across the channel for rapid sequencing. The electrodes may be fixed to th...
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Systems and methods for protein engineering. The systems include a sequence testing subsystem and a machine learning subsystem. The sequence testing subsystem is configured to express proteins and tes...
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Metabolomics, or the systematic study of all metabolites in an organism, is one of the fastest growing areas of scientific research. Nuclear magnetic resonance (NMR) spectroscopy is one tool used to a...
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‘Nanopore sequencing’ allows a single molecule such as DNA to be analyzed without conventional tools like chemical labels or costly optical instruments. In the process, DNA strands and a stream of...
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Humans are diploid organisms whose (non-sex) cells contain two copies of each chromosome – one from each parent. Each of these two copies may be distinguished by genetic variations such as insertion...
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Proteomics is a relatively new discipline that is a large scale study of proteins. It includes systematically identifying the sequence, characterizing the structure and evaluating the function of all ...
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Protein characterization has become an integral part of modern biology, even inspiring a new discipline known as proteomics, or the classification of proteins based on the genome of an organism.  MS ...
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Tandem mass spectrometry (MS/MS) is a technique capable of identifying large numbers of proteins in complex biological samples. In the process, peptides are ionized and an initial survey scan (i.e., a...
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‘Nanopore sequencing’ holds the potential for sequencing a single molecule of DNA without the need for conventional tools like chemical labels or costly optical instruments. This promising method ...
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Mass spectrometry (MS) is a vital tool for identifying and tallying the different proteins, peptides and other molecules in a sample. To determine relative abundance, reagent tags are used to label th...
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Antibodies are used widely in biological and medical research to bind to and help isolate molecules of interest.  However, the reduced stability and production yields of antibody fragments frequently...
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As resources shift from genome sequencing to understanding and characterizing expressed genes and protein function, reliable and rapid tools for the analysis of protein composition are increasingly vi...
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Quantitative analysis in chemistry is the determination of the absolute or relative abundance of substances present in a sample. For biological samples, quantitative analysis performed via mass spectr...
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In addition to simple nucleotide polymorphisms, the human genome includes intermediate structural variations, such as amplifications, insertions, deletions, inversions and complex rearrangements. Whil...
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Microbial genome sequencing projects uncover large numbers of new genes. Functional analyses of these genes require targeted modifications of particular DNA sequences in their chromosomal locations us...
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Analysis by mass spectrometry requires that molecules first be converted to gas-phase ions. Converting small molecules into ions is straightforward; however, it was not possible to ionize large molecu...
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