There are provided computer systems for visualizing and annotating single molecule images. Annotation systems in accordance with this disclosure allow a user to mark and annotate single molecules of i...
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There is provided a high throughput automated single molecule image collection and processing system that requires minimal initial user input. The unique features embodied in the present disclosure al...
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System, process and software arrangement produces high resolution, high accuracy, ordered, genome wide haplotyped maps from single molecule based approximate ordered maps and the location of genes res...
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A method and system are provided for comparing ordered segments of a first DNA restriction map with ordered segments of a second DNA restriction map to determine a level of accuracy the first DNA map ...
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Constructs, vectors, and methods for enhancing homology directed repair using the CRISPR/Cas9 gene editing platform are disclosed.
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Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavi...
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The disclosure relates to laboratory and bioinformatics methods for isolating, detecting, and characterizing microbes in biological samples using metagenomic approaches.
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Described is a method of synthesizing nucleic acids on polyester substrates and the resulting compositions of matter. The method synthesizes nucleic acids from surface hydroxyl initiation points prese...
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Systems and methods for identifying sequence information from measurements made on single nucleic acid molecules are disclosed. The systems and methods can include binding portions of nucleic acid mol...
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An expression cassette for gene silencing and replacement, including, in operable communication, a promoter, an expression attenuator, a nucleotide sequence encoding a gene for a replacement target pr...
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Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be con...
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A method (100) can be provided to determine the throughput speed v of at least one pore (1), comprising: • feeding (110), using a driving force F, a filiform calibration element (2) through the pore...
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With the explosion of interest in using the CRISPR/Cas9 system for gene editing, there is a strong focus on developing tools for delivering the CRISPR machinery safely and efficiently into cells. Exis...
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Biochemical research and biotechnology rely on nucleic acids; however, nucleic acids frequently encounter nucleases that can negatively affect experiments. Although nuclease inhibitors are often used ...
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New tools for human gene editing are rapidly advancing all sectors of biomedicine from basic biology to somatic gene therapy in the clinic. Precise editing of DNA sequences in the human genome can be ...
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Extraction and isolation of nucleic acids from biological samples is an essential prerequisite for further amplification, detection and quantification of specific genetic sequences. The first step in ...
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Titin is a sarcomeric protein expressed primarily in striated muscles. It is responsible for maintaining the structure and biomechanical properties of muscle cells. In the heart, cardiac titin under...
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Polymerase chain reaction (PCR) is used to generate copies of a particular DNA sequence. The technique is common and indispensable in many research applications. Unfortunately, methods for isolating/p...
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Enzymes called ribonucleotidyl transferases (NTases) add nucleotides to the ends of RNAs. At present only a few such enzymes are known, such as poly(A) polymerases and poly(U) polymerases. The selecti...
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‘Nanopore sequencing’ allows a single molecule such as DNA to be analyzed without conventional tools like chemical labels or costly optical instruments. In the process, DNA strands and a stream of...
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Synthesizing DNA and genes is an important tool in biotechnology. It has become commonplace to synthesize short DNA chains, called oligonucleotides, on a substrate and use these as building blocks to ...
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Synthesizing DNA is an important part of biotechnology but current methods are useful only for relatively short gene sequences. Researchers are forced to construct larger genes through time-consuming ...
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To save time and costs, many alternatives to conventional DNA sequencing have been proposed. One approach utilizes an array of oligonucleotide probes (short nucleic acid chains) synthesized by photoli...
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In animals, plants and other eukaryotic organisms, typically one protein is translated from each mRNA. Usually, translation can start only at one end of the mRNA strand. In contrast, several viruses ...
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Genome editing has proven difficult in cells, especially mammalian cells. One way to improve efficiency is to introduce a double-strand break (DSB) in the desired DNA region. These DSBs stimulate cell...
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