Wisconsin Alumni Research Foundation

Technology

Automated Imaging System For Single Molecules

There is provided a high throughput automated single molecule image collection and processing system that requires minimal initial user input. The unique features embodied in the present disclosure al...
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David Schwartz, Rodney Runnheim, Daniel Forrest | P02230US

Technology

Inorganic Coatings For The Enhancement Of Chemical Transfection

Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavi...
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William Murphy, Andrew Khalil, Xiaohua Yu, Krishanu Saha, Jared Carlson-Stevermer, Benjamin Steyer | P150078US01

Technology

High-Density Nucleic Acid Arrays On Polyester Substrates

Described is a method of synthesizing nucleic acids on polyester substrates and the resulting compositions of matter. The method synthesizes nucleic acids from surface hydroxyl initiation points prese...
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David Lynn, Matthew Holden, Matthew Carter, Lloyd Smith | P170367US02

Technology

System And Method For En Masse Patterning Of Molecule Structures

Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be con...
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David Schwartz, Kristy Kounovsky-Shafer, Juan Hernandez-Ortiz, Konstantinos Potamousis, Kyubong Jo, Juan DePablo, Theo Odijk | P170209US02

Technology

A Method To Determine The Throughput Speed Of A Pore

A method (100) can be provided to determine the throughput speed v of at least one pore (1), comprising: • feeding (110), using a driving force F, a filiform calibration element (2) through the pore...
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Robert Blick, Paul Gwozdz, Abhishek Bhat | P160202US02

Technology

Nanocapsule Delivery System For Ribonucleoproteins

With the explosion of interest in using the CRISPR/Cas9 system for gene editing, there is a strong focus on developing tools for delivering the CRISPR machinery safely and efficiently into cells. Exis...
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Shaoqin Gong, Krishanu Saha, Guojun Chen, Amr Abdeen, Yuyuan Wang, Ruosen Xie | P170376US02

Technology

Nuclease Inhibitors and Methods for Their Use

Biochemical research and biotechnology rely on nucleic acids; however, nucleic acids frequently encounter nucleases that can negatively affect experiments. Although nuclease inhibitors are often used ...
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Ronald Raines, Bryan Smith, David Lynn, Matthew Soellner | P05096US

Technology

S1mplex: A New Tool for Precision Gene Editing

New tools for human gene editing are rapidly advancing all sectors of biomedicine from basic biology to somatic gene therapy in the clinic. Precise editing of DNA sequences in the human genome can be ...
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Krishanu Saha, Jared Carlson-Stevermer, Amr Abdeen, Lucille Kohlenberg | P170309US02

Technology

Titin Minigene: Cardiac Research Tool

Titin is a sarcomeric protein expressed primarily in striated muscles. It is responsible for maintaining the structure and biomechanical properties of muscle cells. In the heart, cardiac titin under...
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Marion Greaser, Shijun Li, Wei Guo | P170288US01

Technology

Streamlined Design for Transferring Analytes

Polymerase chain reaction (PCR) is used to generate copies of a particular DNA sequence. The technique is common and indispensable in many research applications. Unfortunately, methods for isolating/p...
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David Beebe, Ben Casavant, David Guckenberger, Scott Berry | P130361US01

Technology

Poly(UG) Polymerase: A Useful New RNA Tool

Enzymes called ribonucleotidyl transferases (NTases) add nucleotides to the ends of RNAs. At present only a few such enzymes are known, such as poly(A) polymerases and poly(U) polymerases. The selecti...
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Marvin Wickens, Melanie Preston | P140268US02

Technology

Nanopore Antennas for Ultrahigh Speed DNA Sequencing

‘Nanopore sequencing’ allows a single molecule such as DNA to be analyzed without conventional tools like chemical labels or costly optical instruments. In the process, DNA strands and a stream of...
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Robert Blick, Abhishek Bhat, Paul Gwozdz | P140109US01

Technology

Faster, Cheaper DNA Synthesis

Synthesizing DNA and genes is an important tool in biotechnology. It has become commonplace to synthesize short DNA chains, called oligonucleotides, on a substrate and use these as building blocks to ...
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Michael Sussman, Kathryn Richmond, Matt Rodesch | P04400US02

Technology

DNA Synthesis with Error Reduction

Synthesizing DNA is an important part of biotechnology but current methods are useful only for relatively short gene sequences. Researchers are forced to construct larger genes through time-consuming ...
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Peter Belshaw, Michael Sussman, Franco Cerrina, James Kaysen, Brock Binkowski, Kathryn Richmond | P05071US

Technology

Synthesis of High-Density Oligomer Microarrays

To save time and costs, many alternatives to conventional DNA sequencing have been proposed. One approach utilizes an array of oligonucleotide probes (short nucleic acid chains) synthesized by photoli...
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Franco Cerrina, Viktoriya Golovkina | P05183US

Technology

New Protein Production Strategy for Plants

In animals, plants and other eukaryotic organisms, typically one protein is translated from each mRNA. Usually, translation can start only at one end of the mRNA strand. In contrast, several viruses ...
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Aurelie Rakotondrafara, Jincan Zhang | P140069US02

Technology

Gene Editing Tool Uses New Enzyme

Genome editing has proven difficult in cells, especially mammalian cells. One way to improve efficiency is to introduce a double-strand break (DSB) in the desired DNA region. These DSBs stimulate cell...
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James Thomson, Zhonggang Hou, Yan Zhang, Rakhi Rajan, Erik Sontheimer, Alfonso Mondragón | P130315US02

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