Catalysts play countless roles in modern society including production of fertilizer, synthesis of plastics and other ubiquitous materials and development of pharmaceuticals. However, many catalysts re...
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UW-Madison researchers have developed a titin DNA construct (a ‘titin minigene plasmid’) that is useful for identifying agents that modulate the titin size reduction that correlates with a...
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Lactic acid bacteria constitute a large group of Gram-positive bacteria (over 200 species described), of which Lactobacillus is the largest genus in the lactic acid bacteria group. Members of the Lact...
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There is provided a high throughput automated single molecule image collection and processing system that requires minimal initial user input. The unique features embodied in the present disclosure al...
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There are provided computer systems for visualizing and annotating single molecule images. Annotation systems in accordance with this disclosure allow a user to mark and annotate single molecules of i...
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System, process and software arrangement produces high resolution, high accuracy, ordered, genome wide haplotyped maps from single molecule based approximate ordered maps and the location of genes res...
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A method and system are provided for comparing ordered segments of a first DNA restriction map with ordered segments of a second DNA restriction map to determine a level of accuracy the first DNA map ...
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The disclosure relates to laboratory and bioinformatics methods for isolating, detecting, and characterizing microbes in biological samples using metagenomic approaches.
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A method (100) can be provided to determine the throughput speed v of at least one pore (1), comprising: • feeding (110), using a driving force F, a filiform calibration element (2) through the pore...
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Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be con...
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Constructs, vectors, and methods for enhancing homology directed repair using the CRISPR/Cas9 gene editing platform are disclosed.
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An expression cassette for gene silencing and replacement, including, in operable communication, a promoter, an expression attenuator, a nucleotide sequence encoding a gene for a replacement target pr...
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Systems and methods for identifying sequence information from measurements made on single nucleic acid molecules are disclosed. The systems and methods can include binding portions of nucleic acid mol...
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With the explosion of interest in using the CRISPR/Cas9 system for gene editing, there is a strong focus on developing tools for delivering the CRISPR machinery safely and efficiently into cells. Exis...
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Biochemical research and biotechnology rely on nucleic acids; however, nucleic acids frequently encounter nucleases that can negatively affect experiments. Although nuclease inhibitors are often used ...
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New tools for human gene editing are rapidly advancing all sectors of biomedicine from basic biology to somatic gene therapy in the clinic. Precise editing of DNA sequences in the human genome can be ...
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Extraction and isolation of nucleic acids from biological samples is an essential prerequisite for further amplification, detection and quantification of specific genetic sequences. The first step in ...
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Titin is a sarcomeric protein expressed primarily in striated muscles. It is responsible for maintaining the structure and biomechanical properties of muscle cells. In the heart, cardiac titin under...
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Polymerase chain reaction (PCR) is used to generate copies of a particular DNA sequence. The technique is common and indispensable in many research applications. Unfortunately, methods for isolating/p...
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Enzymes called ribonucleotidyl transferases (NTases) add nucleotides to the ends of RNAs. At present only a few such enzymes are known, such as poly(A) polymerases and poly(U) polymerases. The selecti...
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‘Nanopore sequencing’ allows a single molecule such as DNA to be analyzed without conventional tools like chemical labels or costly optical instruments. In the process, DNA strands and a stream of...
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To save time and costs, many alternatives to conventional DNA sequencing have been proposed. One approach utilizes an array of oligonucleotide probes (short nucleic acid chains) synthesized by photoli...
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In animals, plants and other eukaryotic organisms, typically one protein is translated from each mRNA. Usually, translation can start only at one end of the mRNA strand. In contrast, several viruses ...
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Genome editing has proven difficult in cells, especially mammalian cells. One way to improve efficiency is to introduce a double-strand break (DSB) in the desired DNA region. These DSBs stimulate cell...
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Humans are diploid organisms whose (non-sex) cells contain two copies of each chromosome – one from each parent. Each of these two copies may be distinguished by genetic variations such as insertion...
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