Wisconsin Alumni Research Foundation


UW Technologies Developed Through the Great Lakes Bioenergy Research Center



Translation-Coupling Cassette for Quickly and Reliably Monitoring Protein Translation in Host Cells

Bacterial production of recombinant proteins is used commonly by researchers and commercial entities to manufacture a large variety of proteins. However, many proteins are not produced efficiently in ...

High-Yielding Method for Converting Biomass to Fermentable Sugars for Biofuel Production

Lignocellulosic biomass is a very desirable feedstock for biofuel production.  If the fermentation process for lignocellulose could be optimized, conversion of this biomass could yield 25 to 50 b...

Fatty Acid-Producing Microbes for Generating Medium- and Long-Chain Hydrocarbons

New, renewable sources of transportation fuel are needed to meet continuing demand. While the main focus has been on biomass-derived gasoline alternatives such as ethanol and other short-chain alcohol...

Ethanol Tolerant Yeast for Improved Production of Ethanol from Biomass

Ethanol production from cellulosic biomass can make a significant contribution toward decreasing our dependence on fossil fuels.  However, the fermentation of biomass can be problematic.  On...

Genes for Xylose Fermentation, Enhanced Biofuel Production in Yeast

Efficient fermentation of cellulosic feedstocks is an essential step in the production of biofuel from plant materials. Glucose and xylose are the two most abundant monomeric carbohydrates found in he...

Transgenic Lignin Easier to Break Down for Biofuel

Lignocellulosic biomass is a very desirable feedstock for biofuel production. If the fermentation process could be optimized, conversion of this biomass could yield 25 to 50 billion gallons of ethanol...

Lignin from Transgenic Poplar Is Easier to Process

Lignin is an important plant cell wall component that provides structural support and vascular functions. It is one of the most abundant organic polymers on Earth, constituting about 30 percent of non...

Bacteria Modified to Secrete Biologically Active Protein for Large-Scale Production

E. coli is the most common prokaryote used to produce protein.  The expressed protein generally accumulates in the cytoplasm.  While this approach is useful for some proteins, not a...

Mild, Nontoxic Production of Fuels and Chemicals from Biomass

Fossil fuel resources supply almost 90 percent of the world’s energy and the vast majority of its organic chemicals. This dependency is insupportable in light of rising emissions, demand and diminis...

More Efficient Ethanol Production from Mixed Sugars Using Spathaspora Yeast

Ethanol obtained from the fermentation of grains and sugars is being blended with gasoline to bolster dwindling petroleum supplies. The alcohol increases combustion efficiency and octane value, and ca...

Enhanced Biomass Digestion with Wood Wasp Bacteria

Plant biomass represents a vast and renewable source of energy. However, harnessing this energy requires breaking down tough lignin and cellulose cell walls. In nature, certain microbes can deconstruc...

Modified Microbes Tolerate 50-Fold More Organic Acid

Production of industrial chemicals has long relied on petroleum-based starting material. As reserves of fossil carbon dwindle, a new approach is looking to microorganisms and their ability to convert ...

Microorganisms For Producing Organic Acids


Microorganisms For Producing Organic Acids


Grass Modified for Easier Bioprocessing

Manufacturing paper and producing biofuels is difficult because the lignin in plant cell walls is tough to degrade. Current techniques are energy intensive and use harsh chemicals. In the case of biof...

Salt-Enhanced Production Of Gamma-Valerolactone (Gvl) From Aqueous Solutions Of Levulinic And Formic Acids


Extending Juvenile Stage of Plants for Biofuels and Feedstock

Juvenile and adult grass tissue dramatically differs in anatomy, chemistry and ability to withstand stresses. Juvenile plants cannot flower and their leaves are better able to resist cold and drought....

One- and Two-Phase Conversion of Biomass to Furfural

Exploiting the energy potential of biomass high in cellulose and lignin—including grasses, shrubs, husks, bark, yard and mill offal not readily digestible by humans—offers a vast and renew...

Modified Yeast Ferments Biomass Xylose

Producing biofuel on a useful scale requires efficient fermentation of cellulosic plant material. The sugars glucose and xylose are the most abundant carbohydrates found in hemicellulose. The yeast mo...

Powerful New Enzyme for Transforming Biomass

Converting plant cellulose and hemicellulose into fermentable sugars is a major bottleneck in the biofuel industry. Chemical pretreatment and enzyme hydrolysis (breakdown) usually are required.

Selective Conversion of Lignin into Simple Aromatic Compounds

Lignin is a major component of non-edible biomass (15-30 percent by weight; 40 percent by energy). It is a cheap byproduct of pulp and biofuel production and is one of the few naturally occurring sour...

Better Biomass Conversion with Recyclable GVL Solvent

To recover useful carbohydrates locked in biomass, molecular bonds must be broken while avoiding further reaction of the resulting glucose and xylose sugars. This is a challenge because glucose can de...

Modified Yeast Show Improved Xylose Fermentation and Toxin Tolerance

Bleaching plant material with alkaline hydrogen peroxide (AHP) is an old process used for papermaking. Several decades ago researchers suggested that this method also could be used in biofuel producti...

Gene Controls Flowering Time in Corn

Plant development is marked by three phases: juvenile, adult vegetative and flowering. The timing between phases is known to impact traits like yield, productivity and tissue digestibility. However, t...

Producing Linear Alpha Olefins from Biomass

Linear alpha olefins (LAOs) are valuable commodity chemicals traditionally derived from petroleum. They are versatile building blocks for making a range of chemical products like polyethylene, synthet...

Concentrated C5 and C6 Sugars from Biomass

Widespread production of sustainable fuels and chemicals will require cost-effective methods for breaking down biomass into its constituent sugars. Attaining high yields of concentrated C5 and C6 carb...

Microbes Produce High Yields of Fatty Alcohols from Glucose

Fatty alcohols are used in detergents, emulsifiers, lubricants and personal care items. More than 1.3 million tons of fatty alcohols are used worldwide each year, representing a $3 billion market.

Wisconsin-Sourced Lager Yeast

Beer can be divided into two broad categories: ales and lagers. Ales have been brewed for thousands of years. They are warm fermented (up to 80° F) for as little as three weeks using top-fermenting y...

Modified Yeast to Boost Biofuel Yields

Producing biofuel on an industrial scale requires efficient fermentation of cellulosic plant material. Glucose and xylose are two of the most abundant sugars found in biomass. The yeast most commonly ...

Two-Step Process Converts Lignin into Simple Aromatic Compounds

Lignin is a major component of non-edible biomass. It is a cheap byproduct of pulp and biofuel production and is one of the few naturally occurring sources of valuable aromatic compounds. Converting l...

High-Throughput Genome Editing and Engineering of Industrial Yeast, Other Fungi

As the industrial uses of yeast and other fungi expand, new systems are needed to genetically optimize strains for their intended use. In particular, the ability to manipulate prototrophic and diploid...

Natural Antimicrobial Agent Derived from Biomass

Fungal pathogens pose one of the greatest economic threats to agriculture. Every year fungal infections – such as root rot, smut and powdery mildew – destroy about 125 million tons of the top five...

Modified E. coli for Enhanced Production of Pyruvate, Ethanol

Pyruvate is a starting material for synthesizing a variety of biofuels and chemicals. It can be readily converted into more than 60 commercial chemicals, including ethanol and several active pharmaceu...

Bio-Based Production of Non-Straight-Chain and Oxygenated Fatty Acids for Fuels and More

Fatty acids generally can be classified as straight-chain or non-straight-chain. Non-straight-chain fatty acids are less abundant and highly valued in dietary supplements, cosmetics, pharmaceuticals, ...

Natural Antibiotic to Treat Clostridium Infection and More

Bacterial resistance to antibiotics is an escalating problem, especially in hospital settings. The Centers for Disease Control recently identified Clostridium difficile as posing an “urgent...

Modified Yeast with Enhanced Tolerance for GVL Biomass Solvent

Gamma-valerolactone (GVL) is an inexpensive solvent derived from biomass that can be used to break apart tough lignocellulose into fermentable sugars including xylose and glucose. GVL-based techniques...

High Yield Method to Produce LGO from Biomass

Levoglucosenone (LGO) is a highly dehydrated sugar typically derived from cellulose. It is an important, non-petroleum building block chemical with potential uses in a wide range of industrial process...

Zip-Lignin™ Assay: An Analysis and Validation Tool

To produce pulp from wood, harsh chemicals are applied during cooking and bleaching mainly because lignin is tough to break down. To make it easier to degrade, Wisconsin researchers discovered how che...

Improved Biomass Conversion with GVL Co-Solvent

Production of fuels and chemicals from biomass requires utilization of all biomass fractions, i.e., cellulose, hemi-cellulose and lignin. Thus, product molecules from all fractions of biomass must be ...

Nitroxyl-Mediated Oxidation Of Lignin And Polycarboxylated Products


Polycarboxylated Compounds And Compositions Containing Same


Industrial Streptomyces with Capability to Grow on Cheap and Abundant Cellulose

Many species of Streptomyces are used industrially in the production of medicines, proteins and other fine commodity chemicals. However, the vast majority must be grown on high-cost glucose fee...

‘Green’ Tylenol: Synthesizing Acetaminophen from Renewable Biomass

Paracetamol (acetaminophen) is an analgesic and fever-reducing medicine. It is an active ingredient in many widely sold over-the-counter medicines such as Tylenol and Panadol. As a prescription pharma...

Recombinant High-Lipid Microbe for Bioproduct & Biofuel Production

Lipids derived from microorganisms, including bacteria, yeast and microalgae, offer a promising source of renewable fuels and chemicals. Oleaginous microbes – defined as those accumulating more than...

Microbiomes And Methods For Producing Medium-Chain Fatty Acids From Organic Substrates


Enzymatic Depolymerization of Lignin

Lignin is a renewable resource that accounts for up to 30 percent of the dry weight of vascular plant cell walls. It is comprised of aromatic compounds that may be valuable commodities for the biofuel...

Polypeptide And Yeast Cell Compositions And Methods Of Using The Same


Method Of Selectively Oxidizing Lignin


Microorganisms And Methods For Producing 2-Pyrone-4,6-Dicarboxylic Acid And Other Compounds


Method To Liquefy Biomass


High Yield Method to Produce HMF from Fructose

The platform molecule 5-hydroxymethylfurfural (HMF), produced from the dehydration of C6 sugars (hexoses), is considered to be one of the top value-added chemicals. The efficient production of HMF fro...

Yeast Strains With Selected Or Altered Mitotypes And Methods Of Making And Using The Same


A Ratiometric Biosensor To Measure Intracellular Nadh/Nad+ Redox