UW researchers have developed modified genetically encoded plasma membrane voltage probes for monitoring mitochondrial and ER membrane voltage gradients. A fluorescence protein tag was coupled with a ...
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Described herein is a method of preparing an unbiased library of phage variants,comprising (a) preparing a population of “acceptor phage”; (b) removing an endogenoustarget gene and inserting gene ...
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The present disclosure provides for methods of diagnosing and treating bacterial infections. Human Intelectin 1 (hIntL-1) has been shown to bind selectively to glycan components on bacteria including ...
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The present disclosure provides for methods providing a pre/probiotic agent to a subject. Human Intelectin 1 (hIntL-1) has been shown to bind selectively to glycan components on bacteria, thereby prom...
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R1 and R2 are hydrogen or C1 to C12 linear or branched alkyl; R3 is hydrogen, C1 to C12 linear or branched alkyl, or nitrogen protecting groups; X is oxygen or nitrogen; and when X is oxygen, R4 is ab...
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An apparatus for measuring lesion volume, for example, in laboratory animals, provides a mirror box allowing a camera to simultaneously acquire profiles of the lesion along at least two crossing axes ...
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The disclosure relates to laboratory and bioinformatics methods for isolating, detecting, and characterizing microbes in biological samples using metagenomic approaches.
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Compounds of Formula I: [Image Omitted] wherein: R1 and R1′ are independently selected from hydrogen, halogen, C1-to-C12 linear or branched hydroxyalkyl, C1-to-C12 linear or branched halo-alkyl, ...
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A cyanuric acid-responsive biosensor includes a host strain or cell-free system including a first and a second expression cassette; the first expression cassette including, in operable communication, ...
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Described herein is a redox biosensor system for measurement of NADH/NAD+ ratio including a host strain or cell-free system including first and a second expression cassette; the first expression casse...
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It is estimated that half of new HIV infections in the U.S. are from patients who know they are HIV-positive but underestimate their transmissibility. Identifying and monitoring the latent HIV reservo...
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In nature, most microbes live in synergistic communities (microbiomes) as a way to thrive in various environments. For example, more than 100 trillion bacteria are estimated to live in the human gut a...
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Biosensors to detect small molecule ligands (e.g., metabolites) have applications in synthetic biology, medical diagnosis, environmental monitoring, bioremediation and bioenergy. Protein-based biosens...
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Mass spectrometry is a broadly applicable analysis tool for detecting and characterizing molecules and other chemical species, including complex mixtures of biomolecules such as peptides, proteins or ...
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Mass spectrometry is a highly selective and quantitative technique that is widely used to determine the chemical composition of a sample. Applications of this analytical tool include measuring isotope...
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To produce pulp from wood, harsh chemicals are applied during cooking and bleaching mainly because lignin is tough to break down. To make it easier to degrade, Wisconsin researchers discovered how che...
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Assays currently used to detect neutralizing antibodies to influenza virus or measure virion binding to cells, antibodies or receptors require multiple steps. These steps are destructive, low sensitiv...
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Influenza infections in humans, from the common seasonal flu to the deadly pandemics of the 20th century, are caused chiefly by influenza A and influenza B viruses. Understanding the in vivo dynamics ...
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Regenerative medicine requires a practical, high throughput method of assessing the cellular components of multicellular entities before transplantation. Such a method would have many clinical and res...
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Circulating tumor cells (CTCs) are cells shed from tumor deposits that flow into the bloodstream. They are detected at a frequency of about one CTC per one billion surrounding blood cells. The great h...
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Mass spectrometers are analytical instruments that can precisely measure the mass of molecules to determine their composition and identity. Generally, the molecules are ionized and then accelerated by...
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In the past decade, cataloguing the thousands of proteins present in a cell using mass spectrometry (MS) has matured into a procedure both deeply vital and straightforward. Yet discovering how such mo...
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Currently, it is not possible to visualize the in vivo dynamics of an influenza infection. All existing models rely on proxies to measure viral replication (e.g., viral concentration in nasal washes) ...
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For diagnosis and research it often is necessary to isolate a subset of cells from a sample that contains bodily fluids, contaminants, tissue and other types of cells. For example, determining a pati...
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Surface plasmon resonance (SPR) imaging has demonstrated its ability to monitor interactions between biological moieties in real time without the aid of chemical labels such as fluorophores and radioi...
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