Wisconsin Alumni Research Foundation

Technology

Simplified Basic Media For Human Pluripotent Cells

Fully defined media that support pluripotent cell viability, proliferation, cloning, and derivation, as well as methods and compositions including these media are described. Methods for deriving iPS c...
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James Thomson, Guokai Chen | P120133US01

Technology

Fgf Having Enhanced Stability

Methods are provided that exploit thermostable FGF-1 proteins for support of human pluripotent stem cell cultures. Also provided are compositions containing thermostable FGF-1 for culturing of human p...
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James Thomson, Guokai Chen | P110094US02

Technology

Fgf-2 Having Enhanced Stability

Thermostable FGF-2 proteins having enhanced ability to support human pluripotent stem cell cultures are provided. Also provided are methods and compositions utilizing thermostable FGF-2 proteins.
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James Thomson, Guokai Chen, Zhonggang Hou | P110177US02

Technology

Polymer Coating for Cell Culture Substrates

A stem cell’s microenvironment plays a key role in regulating its behavior (e.g., adhesion, migration, proliferation and differentiation). A variety of templates have been used to study stem cell be...
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Padma Gopalan, William Murphy, Samantha Brittelle | P150079US01

Technology

Clonal Culture of Human Pluripotent Stem Cells

For researchers, cloning human embryonic stem (ES) cells is challenging because it requires growing new colonies from a single cell. One difficulty is very low cloning efficiency – less than 0.1 pe...
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James Thomson, Veit Bergendahl | P08168US

Technology

Microwells for Controlling Embryoid Body Formation

Embryonic stem cells form aggregates, called embryoid bodies (EBs), before differentiating into distinct types of cells. Clumps of stem cells can vary widely in size, shape and volume, resulting in no...
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Sean Palecek, Jeffrey Mohr, Juan DePablo | P06196US

WARF