Diagnostics & Biomarkers
Detection of Low-Abundance Proteins in Biological Samples Using Size-Exclusion Chromatography and Mass Spectrometry
WARF: P250016US03
Inventors: Manish Patankar, Mark Etzel, Naviya Schuster-Little, Rebecca Whelan
The Invention
UW-Madison researchers in collaboration with University of Kansas researchers have developed a blood-based assay capable of quantifying MUC16/CA125 levels in serum. This assay does not use antibodies, instead it uses mass spectrometry for detection which improves the sensitivity and selectivity. In order to reduce signal-to-noise issues that prevent detection of the protein using mass spec, the method uses size exclusion chromatography to remove most of the proteins in the serum. Due to the very large size of MUC16, it elutes quickly. The inventors used disposable columns for this purification step.
Additional Information
For More Information About the Inventors
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