Wisconsin Alumni Research Foundation

Technology

Artificial Transcription Factors and Uses Thereof

Transcription factor expression can profoundly alter the transcriptional dynamics of a cell, even inducing the transition to a new cell fate. Four transcription factors – Oct4, Sox2, Klf4, and c-Myc...
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Aseem Ansari, Asuka Eguchi | P160383US02

Technology

Nanocapsule Delivery System for Ribonucleoproteins

With the explosion of interest in using the CRISPR/Cas9 system for gene editing, there is a strong focus on developing tools for delivering the CRISPR machinery safely and efficiently into cells. Exis...
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Shaoqin Gong, Krishanu Saha, Guojun Chen, Amr Abdeen, Yuyuan Wang, Ruosen Xie | P170376US02

Technology

Nuclease Inhibitors and Methods for Their Use

Biochemical research and biotechnology rely on nucleic acids; however, nucleic acids frequently encounter nucleases that can negatively affect experiments. Although nuclease inhibitors are often used ...
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Ronald Raines, Bryan Smith, David Lynn, Matthew Soellner | P05096US

Technology

S1mplex: A New Tool for Precision Gene Editing

New tools for human gene editing are rapidly advancing all sectors of biomedicine from basic biology to somatic gene therapy in the clinic. Precise editing of DNA sequences in the human genome can be ...
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Krishanu Saha, Jared Carlson-Stevermer, Amr Abdeen, Lucille Kohlenberg | P170309US02

Technology

Enhanced Biomass Digestion with Wood Wasp Bacteria

Plant biomass represents a vast and renewable source of energy. However, harnessing this energy requires breaking down tough lignin and cellulose cell walls. In nature, certain microbes can deconstruc...
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Cameron Currie, Brian Fox, Taichi Takasuka, Adam Book | P110314US03

Technology

Creating ‘Designer’ Yeast Hybrids for Brewing and More

Interspecies yeast hybrids are critical for producing commercially important fermentation products such as Belgian ale, certain ciders and cold-fermented wines. As one major example, lager beer, the m...
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Chris Hittinger, David Peris Navarro, William Alexander | P160107US03

Technology

Reagents for Bioreversible Protein Esterification

Chemoselective transformations are of key importance in modern chemical biology. Protein esterification can, for example, be employed for labeling, enhanced cellular uptake and other useful protein mo...
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Ronald Raines, Kalie Mix | P150262US03

Technology

Titin Minigene: Cardiac Research Tool

Titin is a sarcomeric protein expressed primarily in striated muscles. It is responsible for maintaining the structure and biomechanical properties of muscle cells. In the heart, cardiac titin under...
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Marion Greaser, Shijun Li, Wei Guo | P170288US01

Technology

Wis.L Master Cell Bank for Vaccine Development

FDA-qualified cell lines must be used for virus production for human vaccines. There are relatively few of these lines available and several (WI-38, MRC-5) used for virus production are becoming senes...
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James Gern | P160170US01

Technology

Animal Model of Brittle Bone Disease

Osteogenesis imperfecta (OI) or brittle bone disease is an incurable genetic disorder that causes fragile bones and a lifetime of breaks and fractures. Recently, mutations in the gene for bone morphog...
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Daniel Greenspan, Se-Jin Lee | P160036US01

Technology

Zip-Lignin™ Assay: An Analysis and Validation Tool

To produce pulp from wood, harsh chemicals are applied during cooking and bleaching mainly because lignin is tough to break down. To make it easier to degrade, Wisconsin researchers discovered how che...
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John Ralph, Steven Karlen, Fachuang Lu, Dharshana Padmakshan | P150118US02

Technology

Yeast-Based Intein Platform for Drug Production

The therapeutic and biochemical properties of proteins including antibodies can be enhanced by custom chemical functionalization that enables modifications, such as small molecule drug conjugation, PE...
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Eric Shusta, Cathryn Marshall | P150056US02

Technology

Enhancing Cell Penetration to Improve Drug Delivery

The utility of many biologic drugs is limited by inefficient cellular delivery. There is a particular need for methods and reagents that facilitate delivery of peptides and proteins into the interior ...
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Ronald Raines, Kristen Andersen | P150031US02

Technology

Bovine Macrophage Cell Line

Macrophages are white blood cells that ingest foreign material and play a crucial role in the immune system. Presently, there is no bovine macrophage cell line available for research and testing purpo...
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Charles Czuprynski, Dhammika Atapattu, Josely Figueiredo | P140060US01

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