BLOOD BRAIN BARRIER SELECTIVE ANTIBODIES AND METHODS OF USE
Inventors: Eric Shusta, Charles Stutz
UW-Madison researchers have developed antibodies or antigen binding fragments thereof (i.e., single chain variable fragment (scFv)) that specifically bind to the blood brain barrier (BBB). The researchers used transcardial perfusion to introduce a phage displayed scFv library into rat brain. After multiple rounds of screening, next generation sequencing (NGS) data were generated from phage DNA pools and subsequently analyzed using the Antibody Mining Toolbox. From this 12 scFvs were further analyzed, resulting in two particularly promising clones, scFv 4and scFv 40, that demonstrated exceptional binding to the rat BBB. These scFvs could be conjugated to an active agent (e.g., therapeutic) for targeted delivery to the BBB.
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