Wisconsin Alumni Research Foundation

Animals, Agriculture & Food
Animals Agriculture Food
GENETICALLY MODIFIED NITROGEN FIXING BACTERIA AND USES THEREOF
WARF: P200066US04

Inventors: Jean-Michel Ané, John Peters, Florence Mus, Devanshi Khokhani


The Invention
A genetically modified bacterium for excreting fixed nitrogen (in the form of ammonia) is disclosed. The bacterium can be made by deleting at least a portion of the nifL gene of a diazotrophic y-proteobacterium, and inserting a promoter sequence into the diazotrophic y-proteobacterium genome that is placed and oriented to direct transcription of the rnfl gene complex. The resulting genetically modified bacterium excretes ammonia constitutively and at a greater rate than the wild type bacterium, and can be used to make biofertilizers to stimulate plant growth. The biofertilizers may contain a culture of the bacteria, or a co-culture of the bacteria and a mycorrhizal fungus.
For current licensing status, please contact Mark Staudt at [javascript protected email address] or 608-960-9845

WARF