Titin is a very large cardiac protein that determines the resting tension of the heart in rats. Isoforms of titin are generated by alternative splicing of a single titin gene. Titin in embryonic rat h...
Learn More

Cultured microorganisms produce an extraordinary array of structurally diverse and valuable organic compounds; however, microbes that can be brought into culture by standard techniques represent only ...
Learn More

Three hybrid screens are used to detect RNA-protein interactions.  A problem with three hybrid screens has been high background due to some cDNAs binding and activating transcription in the absence o...
Learn More

The gene HERG1 encodes a potassium-selective ion channel important in maintaining normal cardiac rhythm and cell growth.
Learn More

The liver detoxifies many of the drugs and toxins in blood, which poses a problem in drug development and testing because drugs are rapidly cleared from the system. In the mammalian fetus, the liver i...
Learn More

HERG (human ether-a-go-go-related gene) encodes a potassium ion channel important for maintaining normal cardiac rhythm and cell growth.
Learn More

CpG islands are areas of DNA containing a high CpG content, which often correspond to promoter regions.
Learn More

The human ERG gene encodes a potassium channel that is expressed in the heart. Mutations in the HERG channel are a common cause of long QT syndrome, a disorder associated with delayed cardiac repolari...
Learn More

Cardiac IKr channels are targets associated with inherited and acquired long QT syndrome (LQTS), a disorder that can lead to ventricular arrhythmias. Previously, IKr channels were thought to be compos...
Learn More

The human heart sodium channel beta1 subunit (SCNB1) is a protein encoded by a single gene. It interacts with the alpha subunit to alter the functional properties of the channel. In addition, SCNB1 is...
Learn More

Cultured microorganisms produce an extraordinary array of structurally diverse and valuable organic compounds; however, microbes that can be cultured using standard techniques represent only a small f...
Learn More

Most biochemical interactions are studied in solutions containing dilute concentrations of molecules. However, the complexity of the cellular environment makes it difficult to compare the results of s...
Learn More

No well-characterized prostate stromal cell lines currently exist.
Learn More

The six mammalian actin genes encode a highly conserved family of cytoskeletal protein isoforms important in cell structure, mechanical stability, and motility. Actin isoforms exhibit tissue-specific ...
Learn More

The human either-a-go-go related gene (HERG1 or KCNH2) potassium channel is critical to maintaining normal cardiac rhythm. Because unintended block of HERG channel activity by drugs can cause long QT ...
Learn More

Because they retain large DNA inserts over many generations, bacterial artificial chromosomes (BACs) are popular vectors for genetic and expression analyses of genomic DNA libraries; however, their us...
Learn More

Promoters are genetic regulatory elements that drive gene expression in cells under certain conditions. One way to identify promoters is to use a promoter-trap vector, which is a plasmid containing a ...
Learn More

Microorganisms adapted to grow in exceptionally harsh environments, such as hot springs and arctic soils, are a potentially vast source of novel metabolic processes, antibiotics, enzymes and other pro...
Learn More

Microorganisms adapted to grow in exceptionally harsh environments, such as hot springs and arctic soils, are a potentially vast source of novel metabolic processes, antibiotics, enzymes and other pro...
Learn More

Humans with mutations in the Adenomatous Polyposis Coli (APC) gene develop hundreds or even thousands of adenomas in the colon, which often lead to colorectal cancer. Mouse models of human intestinal ...
Learn More

Actinomycetes, which are Gram-positive filamentous bacteria, produce the majority of known antibiotics. Fungus-growing Attine ants engage in a mutualistic association with actinomycetes to defend thei...
Learn More

Because conventional DNA sequencing using electrophoresis is laborious and time consuming, alternative approaches, such as those using microarrays, have been developed. Photolithographic techniques ar...
Learn More

Functional genomics involves identifying the function of all genes within a sequenced genome. Knockout mutants may be used to identify the in situ function of any gene, without making assumptions abou...
Learn More

Much of the research on RNA transcription is focused on the action of RNA polymerases. Thus, improved methods for purifying and characterizing RNA polymerase greatly enhance studies of transcription. ...
Learn More

Re-polarization of the cardiac action potential, which plays a critical role in maintaining normal cardiac rhythm, occurs mainly due to the action of potassium ion (K+) currents in the heart. The most...
Learn More