Technologies
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WARF: P01395US

Method for Converting Single-Copy BAC Vectors into Conditional High-Copy BAC Vectors


INVENTORS -

Waclaw Szybalski, Jadwiga Wild, Zdenka Hradecna

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing a method for converting a single-copy BAC vector into a conditional high-copy BAC vector.
OVERVIEWSingle-copy bacterial artificial chromosome (BAC) vectors are widely and advantageously used to clone large genomic DNA fragments for subsequent sequence analysis. Due to their single-copy nature, large numbers of cells containing a BAC clone of interest must be grown to produce a sufficient quantity of DNA for analysis.
THE INVENTIONUW-Madison researchers have developed a method for converting a single-copy BAC vector into a conditional high-copy BAC vector. Conversion is achieved by introducing a conditional origin of replication (ori) into a single-copy BAC vector in a host cell. The conditional ori is introduced by site-specific recombination between the single-copy BAC vector and a vector containing the conditional ori, producing a high-copy BAC vector that can be conditionally amplified by activating the ori.
APPLICATIONS
  • Cloning large genomic DNA fragments
KEY BENEFITS
  • Allows easy conversion of existing single-copy BACs into more useful conditionally amplifiable high-copy BAC clones
  • May be used to “retrofit” single-copy BACs, including the widely used pBeloBac11, which contain large inserts for use in high throughput screening
  • Permits production of copious amounts of large genomic fragments from single-copy BAC libraries, without the need for PCR or other amplification techniques
Contact Information
For current licensing status, please contact Jennifer Gottwald at jennifer@warf.org or 608-960-9854.
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