Analytical Instrumentation, Methods & Materials
MODULE TO INTEGRATE SINGLE CELL AUTOFLUORESCENCE LIFETIMES WITH SINGLE-CELL TRANSCRIPTION (SCRNA-SEQ)
WARF: P240227US01
Inventors: Melissa Skala, Kayvan Samimi, Darcie Moore
The Invention
UW-Madison and Morgridge Institute for Research researchers have created the first high throughput instrument to integrate single cell autofluorescence lifetimes with single-cell transcription (scRNA-seq) data. The new module allows cell-to-cell correspondence between high-throughput autofluorescence lifetimes in a flow-based system and scRNA-seq. Specifically, a single cell deposition module interfaces with an autofluorescence flow sorter and deposits single cells into each well of a 384 well plate. The system uses a novel microfluidic channel design required tuning sheath flow rate (80 µL/min), cell flow velocities (2 mm/sec) and dilution factors (<100k cells/mL) to ensure that one cell is present within every 2µL drop from a 34 Ga needle. The timing of single cell events within the autofluorescence lifetime flow sorter is also tuned to optimize photon counts for reliable lifetime recovery (5 ms per cell). Finally, the volume of each drop within each well is controlled to ensure specifications for commercial single cell sequencing (2µL).
Additional Information
For More Information About the Inventors
Tech Fields
For current licensing status, please contact Andy DeTienne at [javascript protected email address] or 608-960-9857