WARF: P130048US01

  • Assigned to WARF as biological material.

Phospho-Specific Antibody to Study Circadian Rhythm Gene PER2


Randal Tibbetts, Naval Shanware

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing antibodies that can detect phosphorylated forms of PER2 protein, which impacts sleep regulation and tumor suppression.
OVERVIEWCircadian rhythms refer to the 24-hour cycles of biological processes that promote daily functioning. The synchronization of an organism’s internal clock to external cues like light and darkness is called entrainment. In mammals, entrainment is critically influenced by the gene known as PERIOD2 (or PER2). Mutation of this gene can lead to lifelong sleep abnormalities. Specifically, mutations can result in differently phosphorylated proteins.

Previous studies have not been able to directly identify PER2 proteins phosphorylated at specific residue sites in vivo. To do so could help clarify the mechanisms and role of PER2 phosphorylation on healthy functioning.
THE INVENTIONUW–Madison researchers have developed phospho-PER2 antibodies that recognize PER2 protein phosphorylated by the enzyme casein kinase 1. The antibodies selectively recognize forms of the protein phosphorylated on Serine-662, -665 and -668.
  • Reagent for studying PER2 regulation in response to circadian and noncircadian cues
  • Only phospho-PER2 antibody in existence
  • Selectively recognizes phosphorylated forms of PER2 in vivo
For More Information About the Inventors
  • Shanware N.P., Hutchinson J.A., Kim S.H., Zhan L., Bowler M.J. and Tibbetts R.S. 2011. Casein Kinase 1-Dependent Phosphorylation of Familial Advanced Sleep Phase Syndrome-Associated Residues Controls PERIOD2 Stability. J. Biol. Chem. 14, 12766 - 12774.
Contact Information
For current licensing status, please contact Jennifer Gottwald at or 608-960-9854.
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