Analytical Instrumentation, Methods & Materials
DIAZOBUTANONE LINKER-ASSISTED HIGH-THROUGHPUT QUANTITATIVE ANALYSIS FOR PHOSPHATE AND SULFATE CONTAINING LIPIDS
WARF: P230446US01
Inventors: Lingjun Li, Ting-Jia Gu, Peng-Kai Liu
The Invention
UW-Madison researchers have developed a novel linker, diazobutanone, for functionalization of phosphate and sulfate containing lipids. The linker, made via a relatively simple two-step process, includes both ketone and diazo functional groups. Mechanistically, the diazo functionality enables direct oxygen-alkylation of lipid phosphate (and sulfate) groups, resulting in oxime bond formation. The derivatized phosphate (or sulfate) group can then be further reacted with existing isobaric mass tags (e.g., aminoxyTMT). Once tagged, traditional mass spectrometry (and associated methods) can be used for multiplexed quantitative analysis of lipids.
Additional Information
For More Information About the Inventors
Tech Fields
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854