Wisconsin Alumni Research Foundation

Analytical Instrumentation, Methods & Materials
Analytical Instrumentation Methods Materials
MODULE TO INTEGRATE SINGLE CELL AUTOFLUORESCENCE LIFETIMES WITH SINGLE-CELL TRANSCRIPTION (SCRNA-SEQ)
WARF: P240227US01

Inventors: Melissa Skala, Kayvan Samimi, Darcie Moore


The Invention
UW-Madison and Morgridge Institute for Research researchers have created the first high throughput instrument to integrate single cell autofluorescence lifetimes with single-cell transcription (scRNA-seq) data. The new module allows cell-to-cell correspondence between high-throughput autofluorescence lifetimes in a flow-based system and scRNA-seq. Specifically, a single cell deposition module interfaces with an autofluorescence flow sorter and deposits single cells into each well of a 384 well plate. The system uses a novel microfluidic channel design required tuning sheath flow rate (80 ┬ÁL/min), cell flow velocities (2 mm/sec) and dilution factors (
Additional Information
For More Information About the Inventors
For current licensing status, please contact Andy DeTienne at [javascript protected email address] or 608-960-9857

WARF