Wisconsin Alumni Research Foundation

Technology

New Disulfide-Bond Reducing Agent

Disulfide bonds between cysteine residues are the most common crosslinking agents in proteins. Reducing these bonds is an ordinary procedure in biochemistry and biotechnology. The most commonly used r...
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Ronald Raines, John Lukesh | P140284US01

Technology

Predicting Protein Hot Spot Residues

Most biological processes involve multiple proteins interacting with each other. While the principles governing protein-protein interactions are not fully understood, it is known that a small subset o...
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Julie Mitchell, Xiaolei Zhu, Steven Darnell | P140357US01

Technology

New Protein Production Strategy for Plants

In animals, plants and other eukaryotic organisms, typically one protein is translated from each mRNA. Usually, translation can start only at one end of the mRNA strand. In contrast, several viruses ...
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Aurelie Rakotondrafara, Jincan Zhang | P140069US02

Technology

Improved Ref Nuclease for Site-Specific DNA Cleavage

Restriction enzymes are commonly used to cleave double-stranded DNA. These enzymes bind to specific sequences of DNA (the ‘restriction site’) and cleave the DNA either at the recognition site or a...
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Michael Cox, Angela Gruber, Tayla Olsen | P130114US02

Technology

Hydrogel Arrays for Screening Cell-Substrate Interactions

While extensive research has been devoted to understanding the influence of medium conditions on cell development, researchers have only recently begun to understand the role of culture surface proper...
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William Murphy, Stefan Zorn, Ngoc Nhi T. Le, Michael Schwartz, Eric Nguyen | P140097US01

Technology

Protecting Groups for Boronic Acids

Boronic acids (i.e., compounds that have a single boron-carbon bond) are one of the most useful functional groups in organic chemistry and chemical biology. They have applications in polymer sciences,...
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Ronald Raines, Brett VanVeller, Matthew Aronoff | P130142US02

Technology

Wisconsin Miniature SwineTM for Biomedical Research

Rodents and other small animals are extensively used as models to study human health and disease, but there are increasing concerns about their relevance. Research funding and regulatory agencies are ...
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Jess Reed, Christian Krueger, Dhanansayan Shanmuganayagam, Thomas Crenshaw, Jamie Reichert, Joan Parrish | P130271US01

Technology

Wisconsin Miniature SwineTM for Cardiovascular Research

Rodents and other small animals are extensively used as models to study human health and disease, but there are increasing concerns about their relevance. Research funding and regulatory agencies are ...
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Jess Reed, Christian Krueger, Dhanansayan Shanmuganayagam, Thomas Crenshaw, Jamie Reichert, Joan Parrish | P140228US01

Technology

Mouse Model for Mania

Presently, there are no good rodent models for mania, which is a critical component of bipolar disorder. The most commonly used approach is to inject normal mice with amphetamines to produce hyperacti...
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Stephen Gammie | P100188US01

Technology

Computer-Based Determination of Haplotype

Humans are diploid organisms whose (non-sex) cells contain two copies of each chromosome – one from each parent. Each of these two copies may be distinguished by genetic variations such as insertion...
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Tom Anantharaman, Bud Mishra, Will Casey | P05170US

Technology

New Amphiphiles for Manipulating Membrane Proteins

Membrane proteins perform many crucial functions in vivo but are difficult to manipulate and study because they are not soluble in simple aqueous buffers. Solubilizing membrane proteins for physical c...
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Samuel Gellman, Pil Seok Chae | P09028US02

Technology

Influenza Reporter Virus Imaged In Vivo

Currently, it is not possible to visualize the in vivo dynamics of an influenza infection. All existing models rely on proxies to measure viral replication (e.g., viral concentration in nasal washes) ...
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Andrew Mehle | P130288US01

Technology

Phosphine Ligands Made Cheaper, Better

Enantiomers are pairs of compounds that have the same chemical formula but different spatial structures (like your left and right hand, they are non-superimposable mirror images of each other). This i...
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Jennifer Schomaker, Robert Grigg, Ryan Van Hoveln | P130268US02

Technology

Designing Ubiquitin Oligomers

Ubiquitin (Ub) is a small protein composed of 76 amino acids and found throughout the cells of eukaryotic organisms. It folds into a compact globular structure, often as part of a complex of proteins....
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Eric Strieter, Ellen Valkevich, Robert Guenette | P120240US02

Technology

Improved Disulfide-Bond Reducing Agents

Disulfide bonds between cysteine residues are the most common crosslinking agents in proteins. Reducing these bonds is an ordinary procedure in biochemistry and biotechnology. The most commonly used r...
Learn More
Ronald Raines, John Lukesh | P110330US02

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