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317 Results for 'Research Tools'
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Technology
Microtube Scaffold for Sensing and Stimulating Nerve Cell Connections
Studying how neurons and other cells work may require stimulating individual or small clusters of cells. The cells conventionally are stimulated through the use of fine electrodes inserted into or nea...
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Robert Blick, Justin Williams, Minrui Yu, Yu Huang | P100158US01
Technology
Traceless Staudinger Ligation for the Synthesis of Peptides and Proteins in Water
UW–Madison researchers previously developed a method of using traceless Staudinger ligation to chemically synthesize proteins (see WARF reference number P00315US). This method is based on ...
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Ronald Raines, Matthew Soellner, Annie Tam | P08350US02
Technology
Nanoscale and Microscale Wireless Stimulating Probes Precisely Deliver Electrical Current to Cells
Studying how neurons and other cells work may require stimulating individual or small clusters of cells. The cells conventionally are stimulated through the use of fine electrodes inserted into ...
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Robert Blick, Max Lagally | P07076US
Technology
Synthesis of Low-Cost, High-Density DNA Microarrays
A DNA microarray is a collection of microscopic DNA spots arranged on a solid surface via covalent attachment to a chemically suitable substrate. DNA microarrays allow hundreds of thousands of DNA seq...
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Franco Cerrina, Omar Negrete | P08067US
Technology
Blue-Green Phytochrome-Based Fluorophores with Strong Fluorescence
Several fluorescent reporter molecules, such as the Green Fluorescent Protein (GFP), are available as research tools to monitor gene activity and protein distribution within cells. However, curr...
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Andrew Ulijasz, Richard Vierstra | P08462US02
Technology
Method and Compositions for Detecting Botulinum Neurotoxin
Botulinum neurotoxins produced by Clostridium botulinum are the most potent toxins known. Because of the threat of bio-terrorism, sensitive methods for detecting botulinum neurotoxin are needed; howev...
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Edwin Chapman, Min Dong | P04086US
Technology
Deposition of Samples and Sample Matrix for Enhanced Sensitivity of MALDI Mass Spectrometry
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is commonly used to identify and characterize proteins and to sequence DNA. A key step in this process involves creating sample sp...
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Max Lagally, David Barnes, Bradley Larson, Martha Vestling | P04151US
Technology
Efficient Whole Genome Analysis System
In addition to simple nucleotide polymorphisms, the human genome includes intermediate structural variations, such as amplifications, insertions, deletions, inversions and complex rearrangements. Whil...
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David Schwartz, Konstantinos Potamousis, Shiguo Zhou, Steven Goldstein, Michael Newton, Rodney Runnheim, Daniel Forrest, Christopher Churas | P07298US
Technology
Modified Strains PJ69-7A and PJ69-7B for the Yeast Two-Hybrid System
The yeast two-hybrid system is a powerful technique used to detect protein-protein interactions. The technique has been used to establish physical interactions between genetically identified proteins,...
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Philip James | P00227US
Technology
A Nucleotide Element That Enhances Transcription from Bacterial Promoters
The main form of RNA polymerase in E. coli is Eσ70. One of the recognition elements within the promoter region for Eσ70 is the UP element. This element is situated upstream of the RNA tran...
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Richard Gourse, Shawn Estrem, Tamas Gaal, Wilma Ross | P99222US
Technology
hMREII Antiserum for the Detection of DNA Damage
DNA damage occurs in organisms as a result of exposure to environmental chemicals and radiation. Failure to repair this damage can lead to tumor formation and cancer. Although a gene has been found in...
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John H. J. Petrini | P95198US
Technology
New Tools for Solubilizing, Isolating and Characterizing Membrane Proteins
Membrane proteins perform many crucial functions in vivo. Physical characterization of these proteins, including the determination of three-dimensional crystal structures, could improve fundamen...
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Samuel Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen | P09037US02
Technology
Mouse Expressed Sequence Tags Related to Liver, Skin, Kidney, Thymus, Lung and Palate Toxicology
Accurate monitoring of gene expression is critical to determining those patterns of expression correlating with exposure to certain drugs or toxins. By elucidating these patterns, researchers may be a...
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Christopher Bradfield, Russel Thomas | P01134US
Technology
Markerless Gene Replacement Plasmids for E. coli
Microbial genome sequencing projects uncover large numbers of new genes. Functional analyses of these genes require targeted modifications of particular DNA sequences in their chromosomal locations us...
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Frederick Blattner, Gyorgy Posfai, Vitaliy Kolisnychenko, Zsuzsa Bereczki | P01153US
Technology
Input Feature and Kernel Selection Improves Speed, Accuracy and Efficiency of Support Vector Machine Classification
Support vector machines (SVMs) are powerful tools for classifying data that are often used in data mining operations. An SVM classifies data in extremely large data sets by identifying a linear or non...
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Olvi Mangasarian, Glenn Fung | P03201US
Technology
Common Variants of the Sodium Channel Alpha Subunits
The SCN5A gene encodes a human cardiac sodium (Na+) channel alpha subunit that carries the inward Na+ current in the heart. Three distinct polymorphic SCN5A clones, hH1, hH1a and hH1b, have been isola...
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Jonathan Makielski, Bin Ye, Michael Ackerman | P02284US
Technology
Expression System and Fermentation Processes For Overexpression Of Holo-Acyl Carrier Protein
Acyl carrier protein (ACP) is a small lipid carrier protein involved in fatty acid biosynthesis and in the solubilization of fats to make them metabolically available. The molecule is difficult to obt...
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Brian Fox, John Broadwater, Jeffrey Haas | P97107US
Technology
Transgenic Mice Containing an Antioxidant Response Element-Driven Reporter Gene
Antioxidant response elements (AREs) are small segments of DNA that turn on a wide variety of genes in response to oxidative stress. They are of particular interest in the study of cancer treatment an...
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Jeffrey Johnson, Delinda Johnson | P00333US
Technology
An Epitope Tag for Gentle Immunoaffinity Chromatography
Epitope tagging provides an improved method of protein immunopurification. The technique involves adding a small peptide epitope to a protein of interest through recombinant DNA techniques. The recomb...
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Richard Burgess, Nancy Thompson | P03006US
Technology
Electrospray Ionization Ion Source with Tunable Charge Reduction
Analysis by mass spectrometry requires that molecules first be converted to gas-phase ions. Converting small molecules into ions is straightforward; however, it was not possible to ionize large molecu...
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Lloyd Smith, Brian Frey, Michael Westphall | P05387US
Technology
Transgenic Mouse for Testing Chemotherapeutic Agents
The gene mdm2 is a negative regulator of p53, which plays a key role in apoptosis and is important in chemotherapy. Mice with knockout mutations in mdm2 would be useful in studying this gene; however,...
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Mary Ellen Perry, Susan Mendrysa | P00331US
Technology
Eight Hybridoma Cell Lines Producing Monoclonal Antibodies Against La Crosse Encephalitis Virus
La Crosse virus is an etiologic agent for childhood encephalitis in North America. Structurally, the nucleocapsid of this virus is enclosed by a host-derived lipid envelope holding two virion glycopro...
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Thomas Yuill | P01150US
Technology
Cell Line Stably Expressing the Human Heart Sodium Channel Beta1 Subunit
The human heart sodium channel beta1 subunit is a protein encoded by a single gene. It interacts with the alpha subunit to alter the functional properties of the channel.
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Jonathan Makielski, Bin Ye, Carmen Valdivia | P03206US
Technology
Expression and Purification of Recombinant Rabbit and Human Phosphoglucomutase
Phosphoglucomutase (PGM) is a glycolytic enzyme that converts glucose 1-phosphate to glucose 6-phosphate. This enzyme plays a major role in directing the flow of carbon toward either anabolic or catab...
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John Markley, Ronnie Frederick, Young Kee Chae | P02088US
Technology
Transgenic Rats Expressing Human Placental Alkaline Phosphatase
The ability to unambiguously mark a cell’s genotype is essential for studies in which genetically distinct cell populations must be distinguished from one another in vivo.
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Eric Sandgren, William Kisseberth, Jan Lohse, Phil Soriano | P01322US