Wisconsin Alumni Research Foundation

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Method of Expanding a Host Range of a Bacterial Plasmid
WARF: P04144US

Inventors: Marcin Filutowicz, Jadwiga Wild

The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing mutations that allow the popular cloning vector R6K to expand its bacterial host range.
Plasmid R6K is an antibiotic resistance plasmid of E. coli and a popular cloning vector. The R6K protein pi, which is encoded by the pir gene, plays a key role in R6K replication.
The Invention
UW-Madison researchers have identified mutations in the pir gene that allow R6K to expand its bacterial host range. A single amino acid change in the pir gene allows the establishment of the R6L plasmid in phylogenetically distinct bacterial species, and also substantially increases the copy numbers.
  • Gene cloning and expression studies
Key Benefits
  • Modified plasmid has broader host range than its wild-type counterpart
  • Allows R6K plasmid to be established in Pseudomonas, Zooglea, Agrobacterium, Acinetobacter and Rhodobacter
  • Replicates with much higher copy number in Vibrionaceae, Plesiomonas, Vibrio and Chromobacterium
Additional Information
For More Information About the Inventors
For current licensing status, please contact Andy DeTienne at [javascript protected email address] or 608-960-9857