Method of Expanding a Host Range of a Bacterial Plasmid
Inventors: Marcin Filutowicz, Jadwiga Wild
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing mutations that allow the popular cloning vector R6K to expand its bacterial host range.
Plasmid R6K is an antibiotic resistance plasmid of E. coli and a popular cloning vector. The R6K protein pi, which is encoded by the pir gene, plays a key role in R6K replication.
UW-Madison researchers have identified mutations in the pir gene that allow R6K to expand its bacterial host range. A single amino acid change in the pir gene allows the establishment of the R6L plasmid in phylogenetically distinct bacterial species, and also substantially increases the copy numbers.
- Gene cloning and expression studies
- Modified plasmid has broader host range than its wild-type counterpart
- Allows R6K plasmid to be established in Pseudomonas, Zooglea, Agrobacterium, Acinetobacter and Rhodobacter
- Replicates with much higher copy number in Vibrionaceae, Plesiomonas, Vibrio and Chromobacterium
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