A Monoclonal Antibody Specific for Brain Alpha-Dystroglycan
Inventors: James Ervasti, Erin McDearmon-Blondell, Ariana Combs
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing monoclonal antibody that specifically recognizes brain alpha-dystroglycan from a variety of species.
The transmembrane protein dystroglycan is associated with autosomal recessive muscular dystrophies. Although dystroglycan is glycosylated in a tissue-specific manner, no commercial monoclonal antibodies capable of differentiating between the various tissue-specific glycoforms of alpha-dystroglycan currently exist.
UW-Madison researchers have developed mAb 2238E2D2, an IgG2b isotype monoclonal antibody that specifically recognizes brain alpha-dystroglycan from a variety of species. They developed this monoclonal antibody by immunizing mice with purified alpha-dystroglycan from bovine brain and screening for antibodies that reacted only with brain alpha-dystroglycan. They also created another monoclonal antibody, mAb 2237E2D1, which is specific for brain alpha-dystrogylcan from cows.
- Research on dystroglycan and associated muscular dystrophies
- Provides—for the first time—an antibody that recognizes alpha-dystroglycan in a tissue-specific manner
Stage of Development
Successfully used to identify brain alpha-dystroglycan in Western blot analysis.
- McDearmon E.L., Combs A.C., Sekiguchi A., Fujiwara H. and Servasti J. 2006. Brain a-Dystroglycan Displays Unique Glycoepitopes and Preferential Binding to Laminin-10/11. FEBS Letters. 580:3381-3385.