Research Tools
FAST, EASY, AND DIRECT AZIDYLATION OF BIOMOLECULES IN SOLUTION
WARF: P220072WO01
Inventors: Michael Sussman, Benjamin Minkoff, Jamison Wolfer, Heather Burch
The Invention
UW-Madison researchers have developed a simple method for directly and rapidly attaching azide (N3) groups to proteins in solution, using widely available solvents, to produce covalently modified azidylated proteins that can be ‘clicked’ onto a variety of alkyne-decorated labels, ligands, and other macromolecules.
The inventors have discovered that adding azide and hydrogen peroxide to protein in aqueous systems produces significant azidylation of the proteins, which can then be further modified in any number of ways using click chemistry via the CuAAC reaction (copper catalyzed azide-alkyne cycloaddition). Importantly, the inventors have verified that they can attach azide to protein, click traceable markers onto the azidylated protein, and observe this using Western blotting. Additionally, following click attachment of enrichable moieties onto the azidylated protein, they have observed corresponding mass changes using mass spectrometry.
The inventors have discovered that adding azide and hydrogen peroxide to protein in aqueous systems produces significant azidylation of the proteins, which can then be further modified in any number of ways using click chemistry via the CuAAC reaction (copper catalyzed azide-alkyne cycloaddition). Importantly, the inventors have verified that they can attach azide to protein, click traceable markers onto the azidylated protein, and observe this using Western blotting. Additionally, following click attachment of enrichable moieties onto the azidylated protein, they have observed corresponding mass changes using mass spectrometry.
Additional Information
For More Information About the Inventors
Tech Fields
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854