Fusion Protein Expression Vectors, CMV-GST and CMV-tac-GST
Inventors: William Fahl, T. Herbert Manoharan, Jean Gallo, Andrew Gulick
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in two plasmid vectors that allow expression of the GST pi protein.
Enzymes of the glutathione S-transferase (GST) family are present in both eukaryotes and prokaryotes. As much as 10 percent of the cytosolic protein in some mammalian organs can consist of GSTs.
UW-Madison researchers have developed two plasmid vectors that allow expression of the GST pi protein. These vectors were tested in bacterial and mammalian hosts, where they allowed expression of the GST protein as expected. Pieces of DNA can be inserted into a cloning site in these vectors. The encoded recombinant gene then can be expressed in bacterial and mammalian cells, where it will direct the synthesis of a fusion protein in which the amino terminal portion is a GST and the carboxy terminal end is the protein of interest.
- Useful to create a fusion protein with GST and another protein
- This material is available through the American Type Culture Collection (www.atcc.org) #87629