Bacterial Transcription Enhancers
Inventors: Richard Gourse, Shawn Estrem, Tamas Gaal, Wilma Ross
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing a way to increase gene expression from promoters in bacteria.
Bacterial production of recombinant proteins is used commonly by researchers and commercial entities to manufacture a large variety of proteins.
UW-Madison researchers have developed a way to increase gene expression from promoters in most or all bacterial systems. More specifically, the researchers created a means to increase the rate of transcription initiation from a bacterial promoter by fusing a DNA sequence containing an alpha subunit RNA polymerase binding site to the promoter upstream of the sigma subunit of RNA polymerase binding site. The resulting DNA sequence causes RNA polymerase to bind to the promoter more efficiently, without the need for transcription factors.
- Increasing gene expression from promoters in most, if not all, bacterial systems
- Given that the DNA-binding determinants of the alpha subunit are conserved in nearly all bacteria, this method of increasing transcription should be functional in most or all bacterial systems.
- As many as 12 bacterial strains are available.
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