Bacterial Culture Collection from an Extreme Environment in Alaska
Inventors: Jo Handelsman, Patrick Schloss
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in a culture collection of over 1,000 bacterial isolates from non-permafrost soils in an extremely cold and phosphorus-poor environment.
Microorganisms adapted to grow in exceptionally harsh environments, such as hot springs and arctic soils, are a potentially vast source of novel metabolic processes, antibiotics, enzymes and other proteins. Due to the difficulties involved in culturing these microbes – which by definition possess highly unusual growth requirements – this resource today remains largely untapped.
A team of UW-Madison researchers has now created a culture collection of over 1,000 bacterial isolates from non-permafrost soil in the floodplain of the Tanana River -- an extremely cold and mineral poor environment near Fairbanks, Alaska. To obtain the largest and most diverse collection of microbes possible, the researchers employed a range of media concentrations, added soil extract to the enrichment media, and performed extended incubations at low temperatures. Preliminary screening has identified at least 5 unique isolates with good antibiotic activity. The culture collection is arrayed in 96-well culture plates with 20 percent DMSO for preservation.
- Provides a potentially valuable source of new genes, antibiotics, metabolic processes and cold-adapted enzymes for food processing, medical and industrial applications
- Culture conditions were optimized to obtain the most diverse collection of hard-to-isolate bacteria possible.
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