Wisconsin Alumni Research Foundation

Drug Discovery & Development
Drug Discovery Development
WARF: P200363US03

Inventors: Eric Shusta, Hideaki Nishihara, Benjamin Gastfriend, Sean Palecek, Britta Engelhardt

The Invention
A research collaboration between UW-Madison and the University of Bern has resulted in a new protocol for generating cells that can be used to better model the response and behavior of immune cells in the brain. The protocol is based on endothelial cells derived from human iPSC, which are further differentiated to brain microvascular endothelial-like cells (BMECs) using an extended protocol. The result are BMECs having improved characteristics, including immune phenotype markers (i.e., expression of ICAM-1, P-selectin, and VCAM-1) and desirable barrier properties (i.e., moderate TEER, flat cellular morphology, and preferred junction architecture). In sum, this protocol and resulting cells provide a model that faithfully reproduce the required molecular repertoire needed for the study of immune cell trafficking across the blood-brain barrier (BBB). 
Additional Information
For More Information About the Inventors
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854