Wisconsin Alumni Research Foundation

Drug Discovery & Development
Drug Discovery Development
Plasmids And Cell Lines Expressing The DMPK 3'UTR
WARF: P210221US01

Inventors: Mani Mahadevan, Jeffrey Amack

The Invention
The UW Madison researchers' invention is a set of plasmid constructs and cell lines developed to investigate the effects of RNA toxicity in myotonic dystrophy. The inventor created two constructs linking GFP to the DMPK gene having the normal 5 CTG repeats in the 3’ UTR or 200 CTG repeats in the UTR of the DMPK gene (mutant form). The cell lines were used to confirm RNA toxicity on muscle differentiation. These plasmids and cell lines are potentially useful tools for high throughput drug screenings to determine compounds aimed at treating RNA toxicity.
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854