Wisconsin Alumni Research Foundation

Research Tools
Research Tools
Methods Of Genome Editing Including Controlled Opening Of Chromatin
WARF: P220049US02

Inventors: Krishanu Saha, Kaivalya Molugu

The Invention
UW-Madison researchers developed an improved method of gene editing human iPSCs through CRISPR-Cas. They added the histone deacetylase small molecule inhibitor Trichostatin A (TSA) before addition of the CRISPR-Cas machinery to prime the chromatin before the editing and observed that TSA increased the gene-editing efficiency in iPSCs by ∼3.5 fold. Concentration of TSA affected cell viability (> 25 ng/ml of TSA led to a significant decrease in cell viability), so the inventors used TSA concentrations of 0, 3.13, 6.25, and 12.5 ng/mL in their studies. The inventors used the Saha RNP complex for the gene editing work.
Additional Information
For More Information About the Inventors
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854