Wisconsin Alumni Research Foundation

Therapeutics & Vaccines
Therapeutics Vaccines
WARF: P210169US02

Inventors: Adel Talaat, Shaswath Chandrasekar

The Invention
The present invention relates to novel IBV vaccines containing novel modified S and N antigens from avian coronaviruses. The modifications include:
  1. S has a modified transmembrane domain for better secretion and better humoral immunity
  2. S has a modified furin cleavage site to lock S1 and S2 domains to stabilize S antigen and derive better immunity
  3. S has a foldon trimerization domain for better protein assembly and better immune responses.
  4. Codon optimization of all sequences for better expression in poultry tissues
  5. Added HRV 3C site, 8X His tag and Strep II tag for easy protein purification and subsequent tag removal
In this and the previously disclosed SARS-CoV-2 invention (P210042), the inventors have generated and tested DNA and vector vaccine constructs encoding analogously-modified S and N sequences against human and avian coronaviruses, and found significant protection in murine and poultry models. These protective vaccine constructs also generated robust cellular and humoral immune responses, a strong rationale behind their protection. Finally, they have demonstrated that mucosal immunization serves as a robust approach to deliver these vaccine candidates via intranasal spray or oral routes, which represent very practical approaches for mass vaccination against avian coronavirus. Further, the new vaccine constructs can be used individually or in tandem with other constructs for country-specific or global vaccine applications against avian coronaviruses.
Additional Information
For More Information About the Inventors
For current licensing status, please contact Emily Bauer at [javascript protected email address] or 608-960-9842