Therapeutics & Vaccines
Protein Tyrosine Phosphatase 1B Inhibited Neutrophils, Neutrophil-Dendritic Cell Hybrids and Uses Thereof
Inventors: Igor Slukvin, Anna Huttenlocher, Morgan Giese, Ho Sun Jung, David Bennin
UW-Madison researchers have developed methods for preparing PTP1b-deficient neutrophils, which exhibit improved recruitment and antimicrobial functions. The inventors employed CRISPR/Cas9 to delete PTP1b from iPS cells, and then utilized an existing method (P190225) to differentiate the iPSCs into neutrophils. PTP1b-/- neutrophils displayed increased cellular migration and inflammatory cytokine signaling (e.g., IL-6 and TNF) in response to bacterial stimuli, when compared with wildtype cells.