Therapeutics & Vaccines
CHO Cell Line Expressing HERG-1 Potassium Channels
Inventors: Gail Robertson, Barry Ganetzky, Jeffrey Warmke, Matthew Trudeau, Samuel Breit, Terence Campbell, Bruce Walker, Stella Valenzuela
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing a CHO cell line useful for drug screening.
HERG-1 (human ether-a-go-go-related gene) potassium channels mediate repolarization of cardiac action potentials. Loss of function mutations in hERG-1 can result in long QT syndrome, an inherited disorder that increases an individual’s susceptibility to ventricular arrhythmia and sudden death. Current therapies include the use of beta-blockers, but beta-blockers can fail in children and women; therefore, research into additional pharmacotherapies is needed. Chinese hamster ovary (CHO) cells are an epithelial cell line often used in biological and medical research and have been used in a variety of studies to study pharmacotherapies on hERG-1 channels. In the present invention, CHO cells have an overexpression of hERG-1, which may provide an additional tool to screen drugs and compounds for their effects on hERG-1 channels.
UW-Madison researchers have transfected Chinese hamster ovary (CHO) cells with HERG-1 constructs (see WARF reference number P96132US for background on HERG). Since defects in the cardiac potassium channel encoded by HERG-1 can lead to potentially fatal arrythmias, these cells may be used to screen for drugs that may unintentionally block the channel.
- Provide a stable overexpression cell line for hERG-1 potassium channels in ovaries
- May be used as an alternative to stably transfected human embryonic kidney (HEK293) cells
- Has low interference from endogenous channels
- Useful for screening drugs