Therapeutics & Vaccines
IDENTIFICATION AND APPLICATION OF SELECTIVE CELL SURFACE MARKERS AND ADDITIONAL METHODS FOR HUMAN RED/GREEN CONE PHOTORECEPTOR PRECURSOR ENRICHMENT
WARF: P220285US02
Inventors: David Gamm, Praveen Joseph Susai Manickam, Michael Phillips, Elizabeth Capowski
The Invention
UW-Madison researchers have discovered a cell-surface marker (CD166) that can be used to distinguish (and purify) cells that are immature (early) cone photoreceptor precursor cells (CPPs), which will later differentiate into red/green cones. The inventors utilized a dual reporter (THRB2/TdTomato & NRL/eGFP) ESC line to develop 3D retinal organoids; the expression of GFP under the NRL promoter indicates rod precursors, whereas the expression of tdTomato under the THRB2 promoter indicates cone precursors. By performing RNAseq analyses of these cells at several culture time points, the inventors discovered that a cell surface marker, CD166, is transiently present in immature (early) cone photoreceptor precursor cells (CPPs).
Additional Information
For More Information About the Inventors
Tech Fields
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